斑马鱼体细胞核移植（SCNT）的优化方案

Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT).

作者信息

Prukudom Sukumal, Siripattarapravat Kannika, Poulos William, Cibelli Jose B

机构信息

Center for Agricultural Biotechnology, Kasetsart University, Nakhon Pathom, Thailand.

Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok, Thailand.

出版信息

Methods Mol Biol. 2019;1920:353-375. doi: 10.1007/978-1-4939-9009-2_22.

DOI:10.1007/978-1-4939-9009-2_22

PMID:30737703

Abstract

Zebrafish (Danio rerio) is an established animal model to study developmental biology as well as a wide array of human diseases. Here we describe a protocol for somatic cell nuclear transfer (SCNT). This protocol can be used to introduce genetic modifications in zebrafish and for the study of cell plasticity.

摘要

斑马鱼（Danio rerio）是一种成熟的用于研究发育生物学以及多种人类疾病的动物模型。在此，我们描述一种体细胞克隆技术（SCNT）的实验方案。该方案可用于在斑马鱼中引入基因修饰以及研究细胞可塑性。