Interdisciplinary Program in Environmental Science, Graduate School, Chulalongkorn University, Bangkok, 10330, Thailand.
Environmental Research Institute, Chulalongkorn University, Bangkok, 10330, Thailand.
Environ Sci Pollut Res Int. 2019 Apr;26(10):9851-9860. doi: 10.1007/s11356-018-04103-z. Epub 2019 Feb 8.
The primary objective of this research was to investigate the cadmium (Cd) distribution in Pennisetum purpurem (Napier grass) in the presence of 30 mg/L of Cd and different types and concentrations of chelating agents (ethylenediaminetetraacetic acid disodium dihydrate (EDTA), nitrilotriacetic acid (NTA), and EDTA-NTA mixtures). Plant samples were collected every 15 d during a 105-d experimental period. Accumulation of Cd in each part of the plant was determined using atomic absorption spectrometer (AAS), and the distribution of Cd was determined by laser ablation inductively coupled plasma mass spectrometer (LA-ICP-MS) and synchrotron radiation micro X-ray fluorescence (SR-micro-XRF). The highest concentrations of Cd accumulation of 889 ± 53 mg kg in the underground part (roots) and 265 ± 26 mg kg in the aboveground part (stems and leaves) in the presence of 1:1 M ratio of Cd:EDTA after 30 d of exposure were observed. Plants grown in the presence of either NTA or EDTA-NTA mixtures showed significant lower Cd accumulation levels. The LA-ICP-MS analysis showed that Cd was primarily accumulated in the aboveground part (stems and leaves), especially in the xylem and intercalary meristem. In addition, translocation factor was very low. Thus, P. purpurem could be considered as a candidate plant for cadmium phytostabilization.
本研究的主要目的是研究在 30mg/L 的 Cd 存在下，不同类型和浓度的螯合剂（乙二胺四乙酸二钠二水合物（EDTA）、氮川三乙酸（NTA）和 EDTA-NTA 混合物）对杂交狼尾草（Pennisetum purpureum）中 Cd 分布的影响。在 105d 的实验期间，每隔 15d 采集一次植物样本。使用原子吸收光谱仪（AAS）测定植物各部位对 Cd 的积累，并用激光剥蚀电感耦合等离子体质谱仪（LA-ICP-MS）和同步辐射微 X 射线荧光（SR-micro-XRF）测定 Cd 的分布。结果表明，在 30d 暴露期内，Cd:EDTA 摩尔比为 1:1 时，地下部分（根）中 Cd 的积累浓度最高，为 889±53mg/kg，地上部分（茎和叶）中 Cd 的积累浓度最高，为 265±26mg/kg。在 NTA 或 EDTA-NTA 混合物存在下生长的植物，Cd 的积累水平明显较低。LA-ICP-MS 分析表明，Cd 主要积累在地上部分（茎和叶），尤其是木质部和居间分生组织。此外，转运因子非常低。因此，杂交狼尾草可以被认为是一种用于 Cd 植物稳定化的候选植物。
