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2
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Peptain-1 blocks ischemia/reperfusion-induced retinal capillary degeneration in mice.胃蛋白酶-1可阻止小鼠缺血/再灌注诱导的视网膜毛细血管变性。
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Biochem Biophys Res Commun. 2019 Jan 1;508(1):282-288. doi: 10.1016/j.bbrc.2018.11.024. Epub 2018 Nov 26.
2
Matrix-bound AGEs enhance TGFβ2-mediated mesenchymal transition of lens epithelial cells via the noncanonical pathway: implications for secondary cataract formation.基质结合型 AGEs 通过非经典途径增强 TGFβ2 介导的晶状体上皮细胞间充质转化：对继发性白内障形成的影响。
Biochem J. 2018 Apr 23;475(8):1427-1440. doi: 10.1042/BCJ20170856.
3
The Protective Effects of B-Crystallin on Ischemia-Reperfusion Injury in the Rat Retina.β-晶状体蛋白对大鼠视网膜缺血再灌注损伤的保护作用
J Ophthalmol. 2017;2017:7205408. doi: 10.1155/2017/7205408. Epub 2017 Oct 2.
4
αB-crystallin Expression in Breast Cancer is Associated with Brain Metastasis.αB-晶状体蛋白在乳腺癌中的表达与脑转移相关。
NPJ Breast Cancer. 2015 Oct 21;1:15014-. doi: 10.1038/npjbcancer.2015.14.
5
αB-crystallin is essential for the TGF-β2-mediated epithelial to mesenchymal transition of lens epithelial cells.αB晶状体蛋白对于转化生长因子-β2介导的晶状体上皮细胞上皮-间充质转化至关重要。
Biochem J. 2016 May 15;473(10):1455-69. doi: 10.1042/BCJ20160128. Epub 2016 Mar 17.
6
αB-Crystallin Regulates Subretinal Fibrosis by Modulation of Epithelial-Mesenchymal Transition.αB-晶状体蛋白通过调节上皮-间质转化来调控视网膜下纤维化。
Am J Pathol. 2016 Apr;186(4):859-73. doi: 10.1016/j.ajpath.2015.11.014. Epub 2016 Feb 12.
7
Extracellular α-crystallin protects astrocytes from cell death through activation of MAPK, PI3K/Akt signaling pathway and blockade of ROS release from mitochondria.细胞外α-晶状体蛋白通过激活丝裂原活化蛋白激酶（MAPK）、磷脂酰肌醇-3激酶/蛋白激酶B（PI3K/Akt）信号通路以及阻断线粒体活性氧（ROS）释放来保护星形胶质细胞免于细胞死亡。
Brain Res. 2015 Sep 16;1620:17-28. doi: 10.1016/j.brainres.2015.05.011. Epub 2015 May 18.
8
Alpha B-crystallin improved survival of retinal ganglion cells in a rat model of acute ocular hypertension.αB-晶状体蛋白改善了急性高眼压大鼠模型中视网膜神经节细胞的存活率。
Neural Regen Res. 2012 Jul 5;7(19):1493-7. doi: 10.3969/j.issn.1673-5374.2012.19.008.
9
Alpha B-crystallin correlates with poor survival in colorectal cancer.αB-晶状体蛋白与结直肠癌患者的不良生存率相关。
Int J Clin Exp Pathol. 2014 Aug 15;7(9):6056-63. eCollection 2014.
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Identification of peptides in human Hsp20 and Hsp27 that possess molecular chaperone and anti-apoptotic activities.鉴定人源Hsp20和Hsp27中具有分子伴侣和抗凋亡活性的肽段。
Biochem J. 2015 Jan 1;465(1):115-25. doi: 10.1042/BJ20140837.

一种针对αB-晶体蛋白功能肽的单克隆抗体抑制伴侣和抗凋亡活性。

A monoclonal antibody targeted to the functional peptide of αB-crystallin inhibits the chaperone and anti-apoptotic activities.

机构信息

Sue Anschutz-Rodgers Eye Center and Department of Ophthalmology, University of Colorado School of Medicine, United States.

Sue Anschutz-Rodgers Eye Center and Department of Ophthalmology, University of Colorado School of Medicine, United States; Skaggs School of Pharmacy and Pharmaceutical Sciences, Anschutz Medical Campus, Aurora, CO 80045, United States.

出版信息

J Immunol Methods. 2019 Apr;467:37-47. doi: 10.1016/j.jim.2019.02.004. Epub 2019 Feb 6.

DOI:10.1016/j.jim.2019.02.004
PMID:30738041
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6677275/
Abstract

αB-Crystallin is a member of the small heat shock protein family. It is a molecular chaperone and an anti-apoptotic protein. Previous studies have shown that the peptide (DRFSVNLDVKHFSPEELKVKV, hereafter referred to as peptain-1) from the core domain of αB-crystallin exhibits both chaperone and anti-apoptotic properties similar to the parent protein. We developed a mouse monoclonal antibody against peptain-1 with the aim of blocking the functions of αB-crystallin. The antibody reacted with peptain-1, it did not react with the chaperone peptide of αA-crystallin. The antibody strongly reacted with human recombinant αB-crystallin but weakly with Hsp20; it did not react with αA-crystallin or Hsp27. The antibody specifically reacted with αB-crystallin in human and mouse lens proteins but not with αA-crystallin. The antibody reacted with αB-crystallin in human lens epithelial cells, human retinal endothelial cells, and with peptain-1 in peptain-1-transduced cells. Unlike the commercial antibodies against αB-crystallin, the antibody against peptain-1 inhibited the chaperone and anti-apoptotic activities of peptain-1. The antibody might find use in inhibiting αB-crystallin's chaperone and anti-apoptotic activities in diseases where αB-crystallin is a causative or contributing factor.

摘要

αB- 晶体蛋白是小分子热休克蛋白家族的一员。它是一种分子伴侣和抗凋亡蛋白。先前的研究表明，来自 αB-晶体蛋白核心结构域的肽（DRFSVNLDVKHFSPEELKVKV，以下称为肽 1）具有与母体蛋白相似的伴侣和抗凋亡特性。我们开发了一种针对肽 1 的小鼠单克隆抗体，旨在阻断 αB-晶体蛋白的功能。该抗体与肽 1 反应，与 αA-晶体蛋白的伴侣肽不反应。该抗体强烈反应与人重组 αB-晶体蛋白，但与 Hsp20 反应较弱；它与 αA-晶体蛋白或 Hsp27 不反应。该抗体特异性地与人晶状体蛋白中的 αB-晶体蛋白反应，但不与 αA-晶体蛋白反应。该抗体与人晶状体上皮细胞、人视网膜内皮细胞中的 αB-晶体蛋白反应，与转染肽 1 的细胞中的肽 1 反应。与针对 αB-晶体蛋白的商业抗体不同，针对肽 1 的抗体抑制了肽 1 的伴侣和抗凋亡活性。该抗体可能在抑制 αB-晶体蛋白的伴侣和抗凋亡活性方面有一定的应用，在 αB-晶体蛋白是病因或致病因素的疾病中。