School of Biosciences, Mahatma Gandhi University, Kottayam, 686560 Kerala, India.
Immunol Lett. 2019 Mar;207:36-45. doi: 10.1016/j.imlet.2019.02.001. Epub 2019 Feb 7.
Resolution of inflammation needs effective and timely removal of dead cells and other toxic products of neutrophils, monocytes, and macrophages. In this study, we evaluated the role of monocytes in the clearance of neutrophil extracellular trap (NET) and apoptotic neutrophils in the inflammation site. For this, monocytes were observed microscopically after exposing them with NETs and/or apoptotic bodies. A subset of monocytes exposed to NETs ejected extracellular traps and this was shown to be mediated by proteins like elastase and citrullinated histones present in NET supernatant. Monocytes showed a preference for the internalisation of the apoptotic body when both NET and apoptotic bodies were present in the medium. The study provides new insight into the role of monocytes in the clearance of NET and apoptotic neutrophils and this information may open up a way in formulating therapeutic strategies for accelerating resolution of inflammation.
炎症的消退需要有效且及时地清除中性粒细胞、单核细胞和巨噬细胞的死亡细胞和其他有毒产物。在这项研究中，我们评估了单核细胞在清除中性粒细胞胞外陷阱 (NET) 和炎症部位凋亡中性粒细胞中的作用。为此，在将 NET 和/或凋亡小体暴露于单核细胞后，我们在显微镜下观察单核细胞。一组暴露于 NET 的单核细胞排出细胞外陷阱，这被证明是由 NET 上清液中存在的弹性蛋白酶和瓜氨酸化组蛋白等蛋白质介导的。当培养基中同时存在 NET 和凋亡小体时，单核细胞显示出对凋亡小体内化的偏好。该研究为单核细胞在清除 NET 和凋亡中性粒细胞中的作用提供了新的见解，这些信息可能为制定加速炎症消退的治疗策略开辟了一条道路。
