Unité mixte de Recherche 996-Inflammation, Chemokines and Immunopathology, Institut National de la Santé et de la Recherche Médicale, Université Paris Sud, Université Paris-Saclay, Châtenay-Malabry, France.
Assistance Publique Hopitaux de Paris, Bichat Hospital, Immunology Department, Paris, France; and.
J Leukoc Biol. 2017 Sep;102(3):775-781. doi: 10.1189/jlb.3MA0916-411R. Epub 2017 May 2.
Neutrophil extracellular traps (NETs) are extracellular DNA filaments formed during neutrophil activation. This process, called netosis, was originally associated with neutrophil antibacterial properties. However, several lines of evidence now suggest a major role for netosis in thrombosis, autoimmune diseases, and cancer. We demonstrate here that highly purified human blood monocytes are also capable of extracellular trap (ET) release in response to several stimuli. Monocyte ETs display a morphology analogous to NETs and are associated with myeloperoxidase (MPO), lactoferrin (LF), citrullinated histones, and elastase. Monocyte ET release depends on oxidative burst but not on MPO activity, in contrast to neutrophils. Moreover, we demonstrate procoagulant activity for monocyte ETs, a feature that could be relevant to monocyte thrombogenic properties. This new cellular mechanism is likely to have implications in the multiple pathologic contexts where monocytes are implicated, such as inflammatory disorders, infection, or thrombosis.
中性粒细胞细胞外陷阱 (NETs) 是中性粒细胞活化过程中形成的细胞外 DNA 丝。这个过程称为网脱粒，最初与中性粒细胞的抗菌特性有关。然而，现在有几条证据表明 NETs 在血栓形成、自身免疫性疾病和癌症中起着重要作用。我们在这里证明，高度纯化的人血单核细胞也能够对几种刺激物释放细胞外陷阱 (ET)。单核细胞 ET 呈现出与 NET 相似的形态，与髓过氧化物酶 (MPO)、乳铁蛋白 (LF)、瓜氨酸化组蛋白和弹性蛋白酶相关。单核细胞 ET 的释放依赖于氧化爆发，但不依赖于 MPO 活性，这与中性粒细胞不同。此外，我们证明了单核细胞 ET 的促凝活性，这一特性可能与单核细胞的血栓形成特性有关。这种新的细胞机制可能对单核细胞参与的多种病理情况（如炎症性疾病、感染或血栓形成）具有重要意义。
