Huang Cheng-Shuang, Tan Mei, Zhang Xiang-Mei, Luo Xi, Tian Run-Mei, Su Qiong, Wu Liu-Song, Chen Yan
The Affiliated Hospital of Zunyi Medical College/The Children's Hospital of Guizhou Province , Zunyi 563003, Guizhou Province, China.
The Affiliated Hospital of Zunyi Medical College/The Children's Hospital of Guizhou Province , Zunyi 563003, Guizhou Province, China.E-mial:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2019 Feb;27(1):45-51. doi: 10.7534/j.issn.1009-2137.2019.01.008.
To investigate the expression of STAT3 gene in patients with acute myeloid leukemia and its correlation with clinical characteristics.
The real-time quantitative RT-PCR was used to detect the level of STAT3 mRNA in bone marrow samples from 38 newly diagnosed patients with acute myeloid leukemia(AML), and its relevance with clinical characteristics and prognosis were statistically analyzed. Western blot was employed to detect the STAT3 protein level in AML patients. The bone marrow cells from 15 healthy subjects were used as control.
At the mRNA level, the expression level of STAT3 in the AML group was significantly higher than that in control group (P<0.05). The level of STAT3 in AML group correlated positively with the risk factors of patients (P<0.01，r=0.592）. The STAT3 expression level in the high-risk group was statistically higher than that in the standard-risk group and the control group (P<0.01，P<0.01). Furthermor, there was no statistical difference between the sub-groups of AML (P>0.05). The median survival time of patients in STAT3 low expression group was logner than that in high expression group, but the difference was not statistically significant (P>0.005). The level of STAT3 protein in AML patients was significantly higher than that in control group (P<0.05）.
The STAT3 gene is highly expressed in AML patients, which may be used as a predictor for high-risk of AML.
探讨信号转导和转录激活因子3（STAT3）基因在急性髓系白血病患者中的表达及其与临床特征的相关性。
采用实时定量逆转录聚合酶链反应（RT-PCR）检测38例新诊断急性髓系白血病（AML）患者骨髓标本中STAT3 mRNA水平，并对其与临床特征及预后的相关性进行统计学分析。采用蛋白质免疫印迹法检测AML患者STAT3蛋白水平。选取15名健康受试者的骨髓细胞作为对照。
在mRNA水平上，AML组STAT3表达水平显著高于对照组（P<0.05）。AML组STAT3水平与患者危险因素呈正相关（P<0.01，r=0.592）。高危组STAT3表达水平显著高于标危组和对照组（P<0.01，P<0.01）。此外，AML各亚组之间无统计学差异（P>0.05）。STAT3低表达组患者的中位生存时间长于高表达组，但差异无统计学意义（P>0.005）。AML患者STAT3蛋白水平显著高于对照组（P<0.05）。
STAT3基因在AML患者中高表达，可作为AML高危的预测指标。
