College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science & Technology of China, Hefei, PR China.
Bioresour Technol. 2019 May;279:307-316. doi: 10.1016/j.biortech.2019.02.016. Epub 2019 Feb 4.
This work aims to investigate whether and how TCC affects hydrogen production using both experimental and model approaches. Experimental results showed that the exposure of TCC not only enhanced the hydrogen production yield but also promoted the hydrogen yield potential and hydrogen production rate. The maximum hydrogen production yield and hydrogen production rate increased from 10.1 ± 0.2 to 14.2 ± 0.2 mL/g VSS and 0.09 to 0.13 mL/g VSS·h, respectively, when TCC level increased from 0 to 1403 ± 150 mg/kg TSS. Mechanism exploration showed that the presence of TCC significantly promoted the release of substances and observably facilitated the acidification process but seriously inhibited the methonogenesis and homoacetogenesis processes. Further investigations with enzyme analysis revealed that TCC importantly increased the activities of acetate kinase and [FeFe] hydrogenase but seriously inhibited the activities of carbon monoxide dehydrogenase and Coenzyme F420.
本研究旨在通过实验和模型方法来探究三氯卡班（TCC）是否以及如何影响氢气的生成。实验结果表明，TCC 的暴露不仅提高了氢气的产率，还提高了产氢潜力和产氢速率。当 TCC 水平从 0 增加到 1403±150mg/kg TSS 时，最大氢气产率和氢气产率分别从 10.1±0.2 mL/g VSS 和 0.09 mL/g VSS·h 增加到 14.2±0.2 mL/g VSS 和 0.13 mL/g VSS·h。机理探讨表明，TCC 的存在显著促进了物质的释放，并明显促进了酸化过程，但严重抑制了产甲烷和同型产乙酸过程。进一步的酶分析研究表明，TCC 显著增加了乙酸激酶和[FeFe]氢化酶的活性，但严重抑制了一氧化碳脱氢酶和辅酶 F420 的活性。
