Gomis R, Plaza C, Malaisse W J
Endocrinology and Diabetes Unit, Hospital Clinic, Barcelona, Spain.
Diabetes Res. 1988 Dec;9(4):183-6.
Although several models of experimental diabetes mellitus are currently available, none of them appears to be optimally suited to study the possible deleterious effect of sustained hyperglycemia upon biochemical and functional variables in pancreatic islet cells in the absence of chronic stimulation of secretory activity. In the present study, oral diazoxide administration (0.33 g/Kg body wt. thrice per day for three days) to rats was used to cause both hypoinsulinemia and hyperglycemia (greater than or equal to 10.0 mM), the latter persisting, albeit at a somewhat lower level, for at least 1-2 days after cessation of diazoxide treatment. Ten hours after the last administration of diazoxide, the secretory response of isolated islets to D-glucose over 90 min incubation was not impaired. It is proposed that such a model may help to dissociate the influence of hyperglycemia itself upon islet cell behaviour from the consequences of sustained hypersecretion of insulin.
尽管目前有几种实验性糖尿病模型，但似乎没有一种最适合在不长期刺激分泌活动的情况下，研究持续高血糖对胰岛细胞生化和功能变量可能产生的有害影响。在本研究中，给大鼠口服二氮嗪（0.33 g/Kg体重，每天三次，共三天），以引起低胰岛素血症和高血糖（大于或等于10.0 mM），后者在二氮嗪治疗停止后至少持续1-2天，尽管水平有所降低。在最后一次给予二氮嗪10小时后，分离的胰岛在90分钟孵育期间对D-葡萄糖的分泌反应未受损。有人提出，这样的模型可能有助于将高血糖本身对胰岛细胞行为的影响与胰岛素持续高分泌的后果区分开来。
