Department of Chemical Engineering, Federal University of Santa Maria, UFSM, Santa Maria, Brazil.
Department of Chemical and Food Engineering, Technology Center, Federal University of Santa Catarina, UFSC, Florianópolis, Brazil.
Bioprocess Biosyst Eng. 2019 May;42(5):829-838. doi: 10.1007/s00449-019-02086-w. Epub 2019 Feb 9.
In the present study, it was presented a strategy to maximize the cutinase production by solid-state fermentation from different microorganisms and substrates. The best results were observed using Fusarium verticillioides, rice bran being the main substrate. Maximum yield of cutinase obtained by the strain was 16.22 U/g. For concentration, ethanol precipitation was used, and the purification factor was 2.4. The optimum temperature and pH for enzyme activity were 35 °C and 6.5, respectively. The enzyme was stable at a wide range of temperature and at all pH values tested. The concentrated cutinase was used as an adjuvant in a formulation containing cutinase + bioherbicide. The use of enzyme increased the efficiency of bioherbicide, since cutinase was responsible to remove/degrade the cutin that recovery the weed leaves and difficult the bioherbicide absorption. Cutinase showed to be a promising product to be used in formulation of bioherbicides.
在本研究中，提出了一种通过固态发酵从不同微生物和基质中最大化产脂酶的策略。使用Verticillium 属的稻壳作为主要基质时，观察到了最佳结果。该菌株获得的脂酶最大产量为 16.22 U/g。通过乙醇沉淀进行浓缩，纯化因子为 2.4。酶活性的最适温度和 pH 值分别为 35°C 和 6.5。该酶在很宽的温度范围和所有测试的 pH 值下都稳定。浓缩的脂酶被用作含有脂酶+生物除草剂制剂的助剂。使用酶提高了生物除草剂的效率，因为脂酶负责去除/降解角质，使杂草叶片恢复活力，并使生物除草剂难以吸收。脂酶有望成为生物除草剂制剂中使用的有前途的产品。
