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Purification and structural comparisons of the cytosolic and mitochondrial fumarases from baker's yeast.

作者信息

Boonyarat D, Doonan S

机构信息

Department of Biochemistry, University College, Cork, Ireland.

出版信息

Int J Biochem. 1988;20(10):1125-32. doi: 10.1016/0020-711x(88)90258-3.

DOI:10.1016/0020-711x(88)90258-3
PMID:3073979
Abstract
  1. The cytosolic and mitochondrial fumarases (EC 4.2.1.2) from baker's yeast (Saccharomyces cerevisiae) have been purified to homogeneity. 2. Subunit molecular weights for the cytosolic and mitochondrial isoenzymes were 53,000 and 48,000 respectively. 3. Peptide maps obtained after digestion of the two isoenzymes with trypsin were almost identical but showed significant small differences. The same was true of peptide maps obtained after digestion with the glutamic acid-specific proteinase from S. aureus.
摘要

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引用本文的文献

1
The single translation product of the FUM1 gene (fumarase) is processed in mitochondria before being distributed between the cytosol and mitochondria in Saccharomyces cerevisiae.在酿酒酵母中,FUM1基因(延胡索酸酶)的单一翻译产物在线粒体中进行加工,然后再分布于胞质溶胶和线粒体之间。
Mol Cell Biol. 1994 Jul;14(7):4770-8. doi: 10.1128/mcb.14.7.4770-4778.1994.
2
Evolutionary analysis of aspartate aminotransferases.天冬氨酸转氨酶的进化分析
J Mol Evol. 1995 Apr;40(4):455-63. doi: 10.1007/BF00164031.
3
Inducible overexpression of the FUM1 gene in Saccharomyces cerevisiae: localization of fumarase and efficient fumaric acid bioconversion to L-malic acid.
酿酒酵母中FUM1基因的可诱导过表达:富马酸酶的定位及富马酸高效生物转化为L-苹果酸
Appl Environ Microbiol. 1990 Sep;56(9):2777-83. doi: 10.1128/aem.56.9.2777-2783.1990.