Arina Cut Aria, Amir Darwin, Siregar Yahwardiah, Sembiring Rosita J
Medical Faculty, Universitas Sumatera Utara, North Sumatera, Medan, Indonesia.
Medical Faculty, Universitas Andalas, West Sumatera, Padang, Indonesia.
Open Access Maced J Med Sci. 2019 Jan 4;7(1):29-32. doi: 10.3889/oamjms.2019.026. eCollection 2019 Jan 15.
Stroke is a leading cause of disability and remains the second leading cause of death in the world. Some of the pathogenesis of stroke are interactions between genetic and acquired risk factors, the interaction is related with the atherosclerotic which is the main pathogenesis of ischaemic stroke. Previous studies demonstrated an association between methylene tetra hydro folate reductase (MTHFR) genotype and ischaemic stroke; the genotype is one of the independent risk factor.
This study aims to know about the role of polymorphism gen in ischaemic stroke patients with and without hypertension.
This study is a cross-sectional study, the sample was taken consecutively, after approval by the Medical Faculty Science's Ethics Committee at University Sumatera Utara. All sample matched with inclusion and exclusion criteria, demography data and blood sample were taken. Demography data were analysed using descriptive statistic.
Of the 106 ischaemic stroke patients were divided into two groups, the first group is patients with hypertension (53 patients), and the second group is without hypertension (53 patients). We have done the PCR- RFLP to all the patients, we got 78 patients with of genotype, 23 patients with genotype and 5 patients with genotype. We found polymorphism is more frequent in ischaemic stroke patients with hypertension (16 patients; 69.5%), and all the patient with genotype are an ischaemic stroke with hypertension (5 patients; 100%).
We concluded that polymorphism have an important role in hypertension and ischaemic stroke.
中风是导致残疾的主要原因，在全球仍是第二大致死原因。中风的一些发病机制是遗传和后天危险因素之间的相互作用，这种相互作用与动脉粥样硬化有关，而动脉粥样硬化是缺血性中风的主要发病机制。既往研究表明亚甲基四氢叶酸还原酶（MTHFR）基因分型与缺血性中风之间存在关联；该基因分型是独立危险因素之一。
本研究旨在了解该基因多态性在有或无高血压的缺血性中风患者中的作用。
本研究为横断面研究，样本连续选取，经北苏门答腊大学医学院科学伦理委员会批准。所有样本符合纳入和排除标准，收集人口统计学数据和血样。人口统计学数据采用描述性统计分析。
106例缺血性中风患者分为两组，第一组为高血压患者（53例），第二组为无高血压患者（53例）。我们对所有患者进行了聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP），发现78例患者为 基因型，23例患者为 基因型，5例患者为 基因型。我们发现 多态性在有高血压的缺血性中风患者中更常见（16例；69.5%），所有 基因型患者均为有高血压的缺血性中风患者（5例；100%）。
我们得出结论， 多态性在高血压和缺血性中风中起重要作用。
