Contreras V T, Araujo-Jorge T C, Bonaldo M C, Thomaz N, Barbosa H S, Meirelles M de N, Goldenberg S
Instituto Oswaldo Cruz, Departamento de Bioquímica e Biologia Molecular, Rio de Janeiro, Brasil.
Mem Inst Oswaldo Cruz. 1988 Jan-Mar;83(1):123-33. doi: 10.1590/s0074-02761988000100016.
The biological characterization of the Trypanosoma cruzi clone Dm 28c in terms of its growth in LIT medium, cell-cycle, infectivity to mice and interaction with professional and non-professional phagocytic cells shows that it behaves as a bona fide T. cruzi representant. The biological properties of this myotropic clone do not change according to the origin of the trypomastigote forms (i. e., from triatomines, infected mice, cell-culture or from the chemically defined TAUP and TAU3AAG media). In addition Dm 28c metacyclic trypomastigotes from TAU3AAG medium display a high infectivity level to fibroblasts and muscle cells. Experiments on binding of cationized ferritin to trypomastigotes surface show the existence of cap-like structures of ferritin in regions near the kinetoplast, however the nature and role of these anionic sites remain to be determined. The results indicate that metacyclic trypomastigotes from the Dm 28c clone obtained under chemically defined conditions reproduce the biological behaviour of T. cruzi, rendering this system very suitable for the study of cell-parasite interactions and for the isolation of trypanosome relevant macromolecules.
克氏锥虫克隆Dm 28c在LIT培养基中的生长、细胞周期、对小鼠的感染性以及与专业和非专业吞噬细胞的相互作用方面的生物学特性表明，它表现为真正的克氏锥虫代表株。这种亲肌性克隆的生物学特性不会因锥鞭毛体形式的来源（即来自锥蝽、感染小鼠、细胞培养物或化学限定的TAUP和TAU3AAG培养基）而改变。此外，来自TAU3AAG培养基的Dm 28c循环后期锥鞭毛体对成纤维细胞和肌肉细胞具有高感染性。阳离子化铁蛋白与锥鞭毛体表面结合的实验表明，在动基体附近区域存在铁蛋白帽状结构，然而这些阴离子位点的性质和作用仍有待确定。结果表明，在化学限定条件下获得的Dm 28c克隆的循环后期锥鞭毛体再现了克氏锥虫的生物学行为，使得该系统非常适合用于研究细胞-寄生虫相互作用以及分离锥虫相关大分子。
