Ataturk University, Faculty of Veterinary Medicine, Department of Virology, 25240, Erzurum, Turkey.
Ataturk University, Faculty of Veterinary Medicine, Department of Virology, 25240, Erzurum, Turkey.
Microb Pathog. 2019 Apr;129:131-135. doi: 10.1016/j.micpath.2019.02.010. Epub 2019 Feb 10.
Parvovirus B19 (B19V) is one of the major viral pathogens that infect only human beings. This study's aim is to determine which genotypes of the B19V are present in Turkey and to perform a phylogenetic analysis. Twelve B19V positive serum specimens already diagnosed by real-time PCR amplifying a partial region of the NS gene were included in this study. The serological markers and viral loads of the patients were determined. The positivity of the specimens was confirmed using semi-nested PCR. To determine the genotype of the B19V, PCR-positive amplicons were sequenced directly and compared to GenBank-referenced strain sequences. The phylogeny of the 12 sequenced strains was constructed with the maximum likelihood method. Two different genotypes of B19V were identified in our study. Genotype 2 of B19V was not detected. All of the B19V genotype 1 sequences were clustered in the common genotype 1a cluster (10/12, 83.3%). The average quantification of the B19V strains was determined to be 2.1 × 10 IU/ml. The nucleotide identities between our strains and those isolated in other countries were 85.8%-99.5%. Compared to the Turkish strains identified in our study, at the nucleotide level, the closest strains based on genotypes 3b and 1a were the Germany and Netherlands isolates respectively. This study was the first to provide the genotypic variation of B19V circulated in Turkey. We determined two distinct subtypes of B19V, including subtype 3b and 1a. While the genotype 1 is common all over the world, genotype 3 has begun to spread outside of Africa.
细小病毒 B19（B19V）是仅感染人类的主要病毒病原体之一。本研究旨在确定土耳其存在哪些 B19V 基因型，并进行系统发育分析。本研究纳入了 12 份已通过实时 PCR 扩增 NS 基因部分区域诊断为阳性的 B19V 血清标本。对患者的血清标志物和病毒载量进行了检测。采用半巢式 PCR 进一步确认标本的阳性。为了确定 B19V 的基因型，对 PCR 阳性的扩增子进行直接测序，并与 GenBank 参考株序列进行比较。使用最大似然法构建了 12 个测序株的系统发育树。在本研究中鉴定出两种不同的 B19V 基因型。未检测到 B19V 基因型 2。所有 B19V 基因型 1 序列均聚类在常见的基因型 1a 聚类中（10/12，83.3%）。B19V 株的平均定量为 2.1×10 IU/ml。我们的菌株与其他国家分离的菌株之间的核苷酸同一性为 85.8%-99.5%。与在本研究中鉴定的土耳其菌株相比，在核苷酸水平上，基于基因型 3b 和 1a 的最接近的菌株分别是德国和荷兰分离株。本研究首次提供了在土耳其流行的 B19V 的基因变异情况。我们确定了两种不同的 B19V 亚型，包括 3b 亚型和 1a 亚型。虽然基因型 1 在世界各地都很常见，但基因型 3 已开始在非洲以外传播。
