Hacettepe University, Faculty of Pharmacy, Department of Basic Pharmaceutical Sciences, Ankara, Turkey.
Ankara University, Faculty of Science, Department of Chemistry, Ankara, Turkey.
Eur J Pharm Sci. 2019 Apr 1;131:84-92. doi: 10.1016/j.ejps.2019.02.011. Epub 2019 Feb 8.
In this study, novel elastomeric biodegradable bone regenerative films were developed from metformin (Met) and polyurethane (PU). Metformin was selected due to its osteogenic properties and proper chemical structure to react with PU prepolymer. Metformin was integrated into PU macromolecular structure as chain extender after the synthesis of PU prepolymer via condensation polymerization of polycaprolactone diol and hexamethylene diisocyanate. Chemical, thermal, viscoelastic properties of PU-Met films where characterized and discussed in terms of structure-property relationships. PU-Met films had Tg value around -45 °C and showed superior viscoelastic properties under 1 Hz and 10 Hz tensile oscillation frequencies during dynamic mechanical analysis. On the 21st day of biodegradation studies, PU-Met films degraded 2.3 ± 0.1% and 37.8 ± 4.2% in oxidative and enzymatic media, respectively. Cell-material interactions of elastomeric films were investigated by proliferation (MTT assay), alkaline phosphatase activity (ALP), calcium depositions (Alizarin Red Quantification) and morphological evaluations (SEM). Presence of metformin in PU formulation increased MC3T3-E1 attachment, proliferation and calcium deposition.
在这项研究中，从二甲双胍（Met）和聚氨酯（PU）开发出了新型弹性可生物降解的骨再生薄膜。选择二甲双胍是因为其具有成骨特性和适当的化学结构，可与 PU 预聚物反应。二甲双胍通过聚己内酯二醇和六亚甲基二异氰酸酯的缩聚反应合成 PU 预聚物后，作为扩链剂整合到 PU 大分子结构中。根据结构-性能关系，对 PU-Met 薄膜的化学、热、粘弹性性能进行了表征和讨论。PU-Met 薄膜的 Tg 值约为-45°C，在动态力学分析中 1Hz 和 10Hz 拉伸振荡频率下表现出优异的粘弹性。在生物降解研究的第 21 天，PU-Met 薄膜在氧化和酶促介质中分别降解了 2.3±0.1%和 37.8±4.2%。通过增殖（MTT 测定）、碱性磷酸酶活性（ALP）、钙沉积（茜素红定量）和形态评估（SEM）研究了弹性薄膜的细胞-材料相互作用。PU 配方中存在二甲双胍可增加 MC3T3-E1 附着、增殖和钙沉积。
