孕期母体补充β-羟基-β-甲基丁酸（HMB）会影响新生仔猪卵巢的早期卵泡发生。

Maternal β-hydroxy-β-methylbutyrate (HMB) supplementation during pregnancy affects early folliculogenesis in the ovary of newborn piglets.

作者信息

Hułas-Stasiak Monika, Jakubowicz-Gil Joanna, Dobrowolski Piotr, Tomaszewska Ewa, Muszyński Siemowit

机构信息

Department of Comparative Anatomy and Anthropology, Maria Curie-Sklodowska University, Akademicka St. 19, 20-033, Lublin, Poland.

出版信息

Theriogenology. 2019 Apr 1;128:91-100. doi: 10.1016/j.theriogenology.2019.02.003. Epub 2019 Feb 2.

DOI:10.1016/j.theriogenology.2019.02.003

PMID:30743108

Abstract

Beta-hydroxy-beta-methylbutyrate (HMB) is a leucine metabolite with protein anabolic effects. This study was designed to determine whether prenatal HMB treatment has an effect on oogenesis and folliculogenesis in the ovary of newborn piglets. HMB decreased the number of egg nests and primordial follicles and increased the pool of developing follicles compared to the control group. Although the percentage of TUNEL-positive oocytes within the egg nests was higher in HMB-treated group no increase in the Bax/Bcl-2 ratio and active caspase-3 expression was observed. Moreover, the granulosa cell proliferation index and StAR protein expression were higher in HMB-treated group. In contrast to the control group, the expression of E-cadherins was reduced after the HMB treatment. In addition, a significant increase in the serum level of gonadotropins and steroid hormones was detected in HMB-treated piglets. In conclusion, prenatal HMB treatment dysregulates hormonal homeostasis which impairs early folliculogenesis in piglets.

摘要

β-羟基-β-甲基丁酸酯（HMB）是一种具有蛋白质合成代谢作用的亮氨酸代谢产物。本研究旨在确定产前HMB处理是否对新生仔猪卵巢的卵子发生和卵泡发生有影响。与对照组相比，HMB减少了卵母细胞巢和原始卵泡的数量，并增加了发育中卵泡的数量。尽管HMB处理组卵母细胞巢内TUNEL阳性卵母细胞的百分比更高，但未观察到Bax/Bcl-2比值和活性caspase-3表达增加。此外，HMB处理组颗粒细胞增殖指数和StAR蛋白表达更高。与对照组相比，HMB处理后E-钙黏蛋白的表达降低。此外，在HMB处理的仔猪中检测到促性腺激素和类固醇激素的血清水平显著升高。总之，产前HMB处理会破坏激素稳态，从而损害仔猪的早期卵泡发生。