Knapczyk-Stwora Katarzyna, Grzesiak Malgorzata, Ciereszko Renata E, Czaja Elzbieta, Koziorowski Marek, Slomczynska Maria
Department of Endocrinology, Institute of Zoology and Biomedical Research, Jagiellonian University, Gronostajowa 9, 30-387, Krakow, Poland.
Department of Animal Physiology and Endocrinology, University of Agriculture in Krakow, Al. Mickiewicza 24/28, 30-059, Krakow, Poland.
Theriogenology. 2018 Jun;113:19-26. doi: 10.1016/j.theriogenology.2018.02.008. Epub 2018 Feb 10.
The objective of the study was to examine the effects of androgen and estrogen agonists or antagonists on the follicle formation, ovarian cell proliferation and apoptosis as well as plasma steroid concentration in neonatal pigs. Piglets were injected with testosterone propionate (TP, 20 mg/kg bw), flutamide (FLU, 50 mg/kg bw), 4-tert-octylphenol (OP, 100 mg/kg bw), ICI 182,780 (ICI, 400 μg/kg bw), methoxychlor (MXC, 100 mg/kg bw) or corn oil (CTR, controls) between postnatal Days 1 and 10 (n = 4/group). Heart blood was collected and ovaries were excised from the 11-day-old piglets. The lower percentage of oocytes within an egg nest and higher ovarian expression of active caspase 3 were found in TP (androgen excess) piglets compared to controls. FLU-induced androgen deficiency decreased the percentage of primordial follicles, increased that of early primary follicles and diminished ovarian cell proliferation. OP-induced estrogen action increased the percentage of primordial and developing follicles as well as cell proliferation. ICI-induced estrogen deficiency decreased the percentage of transitional follicles and ovarian cell proliferation, while increased the percentage of primordial follicles and the abundance of active caspase 3. Treatment with MXC, exhibiting estrogenic, antiestrogenic, and antiandrogenic activities, declined the percentage of developing follicles and cell proliferation. Moreover, the investigated compounds differentially affected plasma steroid level. In conclusion, the present study demonstrated clear effects of TP and FLU during the earliest stages of folliculogenesis in pigs (nest breakdown and follicle assembly), whereas OP and ICI influenced also the subsequent stages of follicle initial recruitment and growth. Therefore, the androgen and estrogen seems to be important for the follicle assembly and follicle growth in neonatal porcine ovaries.
本研究的目的是检测雄激素和雌激素激动剂或拮抗剂对新生仔猪卵泡形成、卵巢细胞增殖与凋亡以及血浆类固醇浓度的影响。在出生后第1天至第10天，给仔猪注射丙酸睾酮（TP，20 mg/kg体重）、氟他胺（FLU，50 mg/kg体重）、4-叔辛基苯酚（OP，100 mg/kg体重）、ICI 182,780（ICI，400 μg/kg体重）、甲氧滴滴涕（MXC，100 mg/kg体重）或玉米油（CTR，对照组）（每组n = 4）。采集11日龄仔猪的心脏血液并切除卵巢。与对照组相比，TP（雄激素过量）仔猪的卵母细胞在卵窝内的比例较低，且卵巢中活化的半胱天冬酶3表达较高。FLU诱导的雄激素缺乏降低了原始卵泡的比例，增加了早期初级卵泡的比例，并减少了卵巢细胞增殖。OP诱导的雌激素作用增加了原始卵泡和发育中卵泡的比例以及细胞增殖。ICI诱导的雌激素缺乏降低了过渡卵泡的比例和卵巢细胞增殖，同时增加了原始卵泡的比例和活化的半胱天冬酶3的丰度。MXC具有雌激素、抗雌激素和抗雄激素活性，其处理降低了发育中卵泡的比例和细胞增殖。此外，所研究的化合物对血浆类固醇水平有不同影响。总之，本研究表明TP和FLU在猪卵泡发生的最早阶段（卵窝解体和卵泡组装）有明显作用，而OP和ICI也影响卵泡初始募集和生长的后续阶段。因此，雄激素和雌激素似乎对新生猪卵巢中的卵泡组装和卵泡生长很重要。
