Kitajima S, Koyama T, Yamada Y, Sato F
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan Fax: +81-75-7536398 e-mail:
Graduate School of Biological Science, Nara Institute of Science and Technology (NAIST), Ikoma, Nara 630-0101, Japan, , , , , , JP.
Plant Cell Rep. 1998 Dec;18(3-4):173-179. doi: 10.1007/s002990050552.
The constitutive accumulation of tobacco neutral PR-5 (osmotin-like protein; OLP, PR-5d) in roots and cultured cells was studied in transgenic tobacco plants harboring the OLP promoter::GUS gene. This construct showed strong β-glucuronidase expression in vascular tissues and cortex of roots as well as in cultured cells. Analysis using a mutated promoter showed that ethylene-responsive elements (AGCCGCC) were necessary for constitutive expression in roots and cultured cells. An electrophoretic mobility shift assay indicated that ERF3 (EREBP3), an ethylene-responsive-element-binding factor that was reported to be expressed in roots and in cultured cells as well as in ethephon-treated leaves, could bind to the AGCCGCC sequences of the OLP gene. These findings suggest that AGCCGCC sequences and ERFs mediate the constitutive expression of the OLP gene in roots and cultured cells of tobacco.
在携带OLP启动子::GUS基因的转基因烟草植株中，研究了烟草中性PR-5（类渗透素蛋白；OLP，PR-5d）在根和培养细胞中的组成型积累。该构建体在根的维管组织和皮层以及培养细胞中显示出强烈的β-葡萄糖醛酸酶表达。使用突变启动子的分析表明，乙烯反应元件（AGCCGCC）对于根和培养细胞中的组成型表达是必需的。电泳迁移率变动分析表明，ERF3（EREBP3），一种据报道在根、培养细胞以及乙烯利处理的叶片中表达的乙烯反应元件结合因子，能够与OLP基因的AGCCGCC序列结合。这些发现表明，AGCCGCC序列和ERF介导了OLP基因在烟草根和培养细胞中的组成型表达。
