球子蕨（Matteuccia struthiopteris (L.) Todaro）的快速离体繁殖——一种可食用蕨类植物

Rapid in vitro propagation of Matteuccia struthiopteris (L.) Todaro - an edible fern.

作者信息

Thakur R C, Hosoi Y, Ishii K

机构信息

Bio-Resources Technology Division, Forestry and Forest Products Research Institute, P.O. Box 16, Tsukuba Norinkenkyu Danchi-nai, Ibaraki 305, Japan Fax: +81-298-73-3795 e-mail:

出版信息

Plant Cell Rep. 1998 Dec;18(3-4):203-208. doi: 10.1007/s002990050557.

DOI:10.1007/s002990050557

PMID:30744221

Abstract

A rapid and efficient in vitro plant regeneration method was developed for Matteuccia struthiopteris (L.) Todaro (Ostrich fern). Side shoots, originating in meristems of sectioned rhizomes, were used as explant material. A very high rate of meristem multiplication was achieved by culturing the explants in half-strength MS liquid medium supplemented with 2.0 mg/l N-(4-Pyridyl)-N'-phenylurea (4-PU) and 0.5 mg/l thidiazuron (TDZ). Multiplication of the shoot primordia was faster in suspension culture than on solid medium. Rhizogenesis and growth of regenerants were best achieved on hormone-free one-quarter-strength MS solid medium amended with 0.4% agar and 1.0% activated charcoal. Regenerated plantlets continued to grow after transfer to soil in a phytotron.

摘要

为球子蕨（Matteuccia struthiopteris (L.) Todaro）开发了一种快速高效的体外植物再生方法。源自切段根茎分生组织的侧枝用作外植体材料。通过在添加了2.0 mg/l N-(4-吡啶基)-N'-苯基脲（4-PU）和0.5 mg/l噻苯隆（TDZ）的半强度MS液体培养基中培养外植体，实现了非常高的分生组织增殖率。芽原基在悬浮培养中的增殖比在固体培养基上更快。在添加了0.4%琼脂和1.0%活性炭的无激素四分之一强度MS固体培养基上，再生植株的生根和生长效果最佳。再生苗转移到人工气候箱中的土壤后继续生长。