Piola F, Label P, Vergne P, von Aderkas P, Rohr R
Interactions Plantes Champignons et Micropropagation, Université Lyon 1, F-69622 Villeurbanne Cedex, France e-mail:
INRA, Station d'Amélioration des Arbres Forestiers, F-45160 Olivet, France, , , , , , FR.
Plant Cell Rep. 1998 Dec;18(3-4):279-283. doi: 10.1007/s002990050571.
Axillary and apical buds of in-vitro-propagated cuttings of Cedrus libani are unable to burst at 24 °C, but this inhibition was overcome at 30 °C. Here we have used cedar microcuttings to investigate whether the levels of endogenous hormones vary with bud dormancy and temperature. We analysed the levels of abscisic acid, indole-3-acetic acid, zeatin, isopentenyladenine and their major metabolites using HPLC purification and fractionation of the samples coupled to an ELISA method for hormonal quantitation involving several antibodies elicited against each hormonal family. Abscisic acid levels in microcuttings with dormant buds were higher than those in microcuttings with growing buds. At 24 °C, needles accumulated more abscisic acid than at 30 °C. In addition, when needles were removed, but growth release was achieved at 24 °C. Abscisic acid supplied at 30 °C induced the formation of dormant buds. These results suggest that abscisic acid accumulation in the needles can explain the bud dormancy of cedar microcuttings at 24 °C.
黎巴嫩雪松离体繁殖插条的腋芽和顶芽在24℃时无法萌发，但在30℃时这种抑制作用被克服。在此，我们利用雪松微插条来研究内源激素水平是否随芽休眠和温度而变化。我们使用高效液相色谱法对样品进行纯化和分离，并结合酶联免疫吸附测定法（ELISA）对脱落酸、吲哚-3-乙酸、玉米素、异戊烯腺嘌呤及其主要代谢产物的水平进行分析，该ELISA方法涉及针对每个激素家族产生的几种抗体进行激素定量。带有休眠芽的微插条中脱落酸水平高于带有生长芽的微插条。在24℃时，针叶积累的脱落酸比在30℃时更多。此外，当去除针叶时，在24℃实现了生长释放。在30℃供应脱落酸诱导了休眠芽的形成。这些结果表明，针叶中脱落酸的积累可以解释雪松微插条在24℃时的芽休眠现象。
