Luo M Y, Pan X H, Fan Q, Zhang J F, Ge R, Jiang J, Chen W J
Department of AIDS/STD Prevention and Control, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
Division of AIDS/TB Prevention and Control, Jiaxing Prefectural Center for Disease Control and Prevention, Jiaxing 314001, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2019 Feb 10;40(2):202-206. doi: 10.3760/cma.j.issn.0254-6450.2019.02.015.
To understand the epidemiological characteristics of one large HIV molecular transmission cluster in Jiaxing city, Zhejiang province, 2017 in order to select those people under high-risk and providing basis for programs on prevention. During 2017, newly diagnosed HIV/AIDS cases in this city were recruited. Plasma samples were collected from subjects, followed by RNA extraction, RT-PCR and nest-PCR for gene amplification, before being sequenced and aligned. Mega 6.0 software was used to construct phylogenetic tree, and Cytoscape 3.6.0 software was used to identify HIV molecular transmission clusters. Cases within the large transmission clusters were investigated, using a field-epidemiology-questionnaire. Data related to socio-demographics and previous sexual behaviors were collected and EpiData 3.0 and SPSS 20.0 software were used. In the large transmission cluster with subtype identified as CRF07_BC, in Jiaxing, 2017, 26 cases of the total 30 cases were investigated. A total of 80.8% (21/26) could be identified as newly infected within the last two years and 30.8%(8/26) could be identified as newly infected within the last one year, including 22 cases infected locally. Among several infected cases who were at age 45 years or older, they admitted that they had experienced unprotected sexual contacts in local city for long time and having had more than 10 disclosed sexual contacts within the last two years at the local venues. This molecular cluster had been formed and scaled up quickly in recent two years, it has played an important role in promoting and scaling up the HIV transmission. Three cases identificed as high risk played an importantrde role in scaling up this cluster.
为了解2017年浙江省嘉兴市一个大型HIV分子传播簇的流行病学特征，以便筛选出高危人群并为预防项目提供依据。2017年，招募了该市新诊断的HIV/AIDS病例。采集受试者的血浆样本，随后进行RNA提取、RT-PCR和巢式PCR进行基因扩增，然后进行测序和比对。使用Mega 6.0软件构建系统发育树，使用Cytoscape 3.6.0软件识别HIV分子传播簇。对大型传播簇内的病例进行调查，采用现场流行病学调查问卷。收集与社会人口统计学和既往性行为相关的数据，并使用EpiData 3.0和SPSS 20.0软件。在嘉兴市2017年鉴定为CRF07_BC亚型的大型传播簇中，共调查了30例中的26例。共有80.8%（21/26）可被确定为在过去两年内新感染，30.8%（8/26）可被确定为在过去一年内新感染，其中包括22例本地感染病例。在几名45岁及以上的感染病例中，他们承认在当地长期有不安全性接触，并且在过去两年内在当地场所公开的性接触超过10次。这个分子簇在最近两年迅速形成并扩大规模，在促进和扩大HIV传播方面发挥了重要作用。3例被确定为高危的病例在扩大这个簇的规模方面发挥了重要作用。
