Wan Y H, Zhuang L, Ren L J, Zheng Q N, Fu L, Shan Z Z, Pei F F, Jiang W J, Tang G P, Li S J
Clinical Laboratory of Institute for Communicable Disease Control and Prevention, Guizhou Center for Disease Control and Prevention, Guiyang 550004, China.
Clinical Laboratory of Institute for Communicable Disease Control and Prevention, Qiandongnan Center for Disease Control and Prevention, Kaili 556000, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2019 Feb 6;53(2):229-232. doi: 10.3760/cma.j.issn.0253-9624.2019.02.020.
The number of H7N9 bird flu cases was high and the situation was grim in guizhou province in 2017. To understand the molecular characteristics of the hemagglutinin gene (HA) and the risk of human infection with avian influenza virus A(H7N9) in Guizhou Province, 2017. Homology, genetic evolution and pivotal sites related to receptor binding regions, pathogenicity and potential glycosylation of 14 avian influenza viruses A(H7N9) were analyzed by a series of bioinformation softwares. It was cleared that there was 95.9%-100% similarity among 14 strains in nucleotide of the HA gene, and there were 96.8%-97.8% and 96.8%-97.9% similarities with vaccine strains A/Shanghai/2/2013 and A/Anhui/1/2013 recommended by WHO, respectively. Phylogenetic analysis showed that 14 HA genes were directly evolved in the Yangtze River Delta evolution branch, but they could be derived from five diffenrent strains. Then 13 of 14 strains cleavage site sequences of HA protein revealed they were low pathogenic avian influenza viruses, while A/Guizhou-Weining/CSY01/2017 was high pathogenic avian influenza virus. Mutation G186V at the receptor binding sites in the HA was found in all 14 strains, and mutation Q226L in 13 strains besides A/Guizhou-Weining/CSY01/2017. All five potential glycosylation motifs in the HA were conservative.
2017年贵州省H7N9禽流感病例数较多，形势严峻。为了解2017年贵州省甲型H7N9禽流感病毒血凝素基因（HA）的分子特征及人感染风险，运用一系列生物信息软件对14株甲型H7N9禽流感病毒的同源性、遗传进化以及与受体结合区、致病性和潜在糖基化相关的关键位点进行了分析。结果表明，14株病毒HA基因核苷酸序列相似度为95.9% - 100%，与世界卫生组织推荐的疫苗株A/Shanghai/2/2013和A/Anhui/1/2013的相似度分别为96.8% - 97.8%和96.8% - 97.9%。系统进化分析显示，14个HA基因直接在长江三角洲进化分支中演化，但可能来源于5个不同毒株。14株中有13株HA蛋白裂解位点序列显示为低致病性禽流感病毒，而A/Guizhou-Weining/CSY01/2017为高致病性禽流感病毒。14株病毒HA受体结合位点均发现G186V突变，除A/Guizhou-Weining/CSY01/2017外，13株还有Q226L突变。HA中所有5个潜在糖基化基序均保守。
