Maris E, Ferrieres-Hoa A, Gala A, Coffy A, Vintejoux E, Ranisavljevic N, Hamamah S
Département de médecine de la reproduction, CHU de Montpellier, 34000 Montpellier France; Département de gynécologie obstétrique, CHU de Nîmes, 30000 Nîmes, France.
Département de biologie de la reproduction, CHU de Montpellier, 34000 Montpellier, France; Inserm, U1203, institut de médecine régénératrice et de biothérapie, CHU de Montpellier, 34000 Montpellier, France.
Gynecol Obstet Fertil Senol. 2019 Mar;47(3):305-310. doi: 10.1016/j.gofs.2019.01.011. Epub 2019 Feb 10.
It is already known that children born after slow frozen embryo replacement have a significantly higher birth weight compared to children born after fresh embryo transfer. Similar data have been reported related to frozen embryo transfer using an open vitrification system. However, few data relative to birth weight using a complete embryo closed vitrification system has been reported. The purpose of this study was to know if frozen embryo in closed vitrification system is associated with a higher birth weight compared to fresh embryo replacement.
This was a monocentric retrospective cohort study, 371 children were issued from fresh embryo replacement and 127 from vitrified embryo transfer.
All singletons born after fresh or vitrified embryo transfer between January 2011 and April 2015 were included. Births from the vitrified or fresh transfers of egg or sperm donation, and preimplantation genetic diagnosis were excluded. In addition, pregnancies with more than one gestational sac at the first ultrasound were excluded. An analysis of covariance (ANCOVA) was used for multivariate analysis.
Mean birth weight was 205g higher in the frozen embryo compared with fresh embryos transfer groups (3368g vs. 3163g respectively, P<0.001). This difference remained after multivariate analysis adjusted on confounding factors such as gestational age, maternal age, maternal body mass index (BMI), tobacco exposure, number of embryo transferred and birth order (P<0.001)..
Embryo frozen in closed vitrification system is associated with a higher birth weight compared to fresh embryo replacement. Our findings are consistent with the previous studies related to slow freezing and open vitrification systems data. The effects of controlled ovarian stimulation (COS), ex vivo culture conditions and cryopreservation systems on birth weight of children born should be further explored.
已知与新鲜胚胎移植后出生的儿童相比，慢速冷冻胚胎移植后出生的儿童出生体重显著更高。关于使用开放式玻璃化系统进行冷冻胚胎移植也有类似数据报道。然而，关于使用完整胚胎封闭式玻璃化系统的出生体重数据报道较少。本研究的目的是了解与新鲜胚胎移植相比，封闭式玻璃化系统中的冷冻胚胎是否与更高的出生体重相关。
这是一项单中心回顾性队列研究，371名儿童来自新鲜胚胎移植，127名来自玻璃化胚胎移植。
纳入2011年1月至2015年4月间新鲜或玻璃化胚胎移植后出生的所有单胎。排除卵子或精子捐赠的玻璃化或新鲜移植以及植入前基因诊断后的出生情况。此外，首次超声检查时有多个妊娠囊的妊娠也被排除。采用协方差分析（ANCOVA）进行多变量分析。
冷冻胚胎组的平均出生体重比新鲜胚胎移植组高205g（分别为3368g和3163g，P<0.001）。在对胎龄、产妇年龄、产妇体重指数（BMI）、吸烟暴露、移植胚胎数量和出生顺序等混杂因素进行多变量分析调整后，这种差异仍然存在（P<0.001）。
与新鲜胚胎移植相比，封闭式玻璃化系统中冷冻的胚胎与更高的出生体重相关。我们的研究结果与之前关于慢速冷冻和开放式玻璃化系统数据的研究一致。应进一步探讨控制性卵巢刺激（COS）、体外培养条件和冷冻保存系统对出生儿童出生体重的影响。
