1 Division of Thrombosis Research, Department of Applied Biology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, India.
2 Integrated Cancer Research, Rajiv Gandhi Center for Biotechnology, Thiruvananthapuram, India.
Tissue Eng Part C Methods. 2019 Mar;25(3):148-159. doi: 10.1089/ten.TEC.2018.0333.
With respect to the persistent hunt for a cytocompatible, translational, reproducible, and effective approach in engineering primary human adipose-derived mesenchymal stromal cells (hADMSCs), we demonstrate the application of Neon Transfection System in adequate transient delivery of angiogenic factors. The study presents functional assessment of this approach in vitro, with two notable outcomes at translational perspective; (1) Bioengineered hADMSCs secretome does induce endothelial lineage commitment of stem cells at both transcriptional and translational levels and (2) Combinatorial delivery of vascular endothelial growth factor A and hypoxia-inducible factor-1α by bioengineered hADMSCs enhance upregulation of endothelial cell proliferation, migration-associated wound closure, and endothelial tube formation with augmented Flk-1 expression, as compared with their independent actions. The methods described in this study paves way for in vivo evaluation on identification of appropriate chronic wound models and subsequently for clinical translation. The technology developed also has application in vascularization of tissue-engineered constructs.
关于在工程化原发性人脂肪来源间充质基质细胞（hADMSCs）中持续寻找细胞相容性、可转化、可重现和有效的方法，我们展示了 Neon 转染系统在适当的短暂传递血管生成因子方面的应用。本研究在体外对这种方法进行了功能评估，从转化的角度来看有两个显著的结果；（1）生物工程 hADMSCs 分泌组确实在转录和翻译水平上诱导干细胞向内皮谱系的分化；（2）与它们的独立作用相比，生物工程 hADMSCs 联合递送血管内皮生长因子 A 和缺氧诱导因子-1α 可增强内皮细胞增殖、迁移相关的伤口闭合和内皮管形成，同时增加 Flk-1 的表达。本研究中描述的方法为鉴定合适的慢性伤口模型的体内评估铺平了道路，并随后进行临床转化。所开发的技术也可应用于组织工程构建体的血管化。
