Zhang Lei, Shang Jing, Jia Qi, Li Kai, Yang Hui, Liu Huanhuan, Tang Zhongqin, Chang Xiaoli, Zhang Min, Wang Wenming, Yang Wenyu
Sichuan Engineering Research Center for Crop Strip Intercropping System and Key Laboratory of Crop Eco‑physiology and Farming System in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.
College of Agronomy and Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu, 611130, China.
Arch Virol. 2019 Apr;164(4):1037-1048. doi: 10.1007/s00705-019-04165-5. Epub 2019 Feb 11.
Soybean mosaic virus (SMV) is one of the major pathogens causing serious soybean losses. Little is known about the genetic structure and evolutionary biology of the SMV population in southwestern China. In this study, 29 SMV isolates were obtained from Sichuan Province, and the genomic regions encoding the first protein (P1) and coat protein (CP) were sequenced. Combined with SMV isolates from the southeastern and northeastern regions of China, the genetic and molecular evolution of SMV was studied. Recombination analysis revealed that intraspecific and interspecific recombination had occurred in the SMV population. A phylogenetic tree based on the P1 gene reflected the geographic origin of the non-interspecific recombinant SMV (SMV-NI), while a tree based on the CP gene did not. Though frequent gene flow of the SMV-NI populations was found between the southeastern and northeastern populations, the southwestern population was relatively independent. Genetic differentiation was significant between the SMV interspecific recombinant (SMV-RI) and the non-interspecific recombinant (SMV-NI) populations. It was interesting to note that there was an almost identical recombination breakpoint in SMV-RI and Watermelon mosaic virus (WMV). Population dynamics showed that SMV-RI might be in an expanding state, while the SMV-NI population is relatively stable.
大豆花叶病毒（SMV）是导致大豆严重减产的主要病原体之一。关于中国西南部SMV群体的遗传结构和进化生物学知之甚少。在本研究中，从四川省获得了29个SMV分离株，并对编码第一个蛋白（P1）和外壳蛋白（CP）的基因组区域进行了测序。结合来自中国东南部和东北部地区的SMV分离株，研究了SMV的遗传和分子进化。重组分析表明，SMV群体中发生了种内和种间重组。基于P1基因的系统发育树反映了非种间重组SMV（SMV-NI）的地理起源，而基于CP基因的系统发育树则没有反映。尽管发现SMV-NI群体在东南部和东北部群体之间频繁发生基因流动，但西南部群体相对独立。SMV种间重组体（SMV-RI）和非种间重组体（SMV-NI）群体之间的遗传分化显著。值得注意的是，SMV-RI和西瓜花叶病毒（WMV）存在几乎相同的重组断点。群体动态表明，SMV-RI可能处于扩张状态，而SMV-NI群体相对稳定。
