Holý Ondřej, Matoušková Ivana, Zatloukalová Simona, Dvořáčková Martina, Petrželová Jana, Raida Luděk, Růžička Filip
Department of Public Health, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic, e-mail:
Klin Mikrobiol Infekc Lek. 2018 Sep;24(3):68-72.
Staphylococcus epidermidis and coagulase-negative staphylococci generally are important causative agents of hospital-acquired infections. A significant role in this process is played by their common ability to form biofilm, a highly organized community of microorganisms adhering to inert surfaces. The study aimed to determine the prevalence of these bacterial strains and their ability to form biofilm at the Department of Hemato-Oncology, University Hospital Olomouc.
Over a period of 12 months, samples of air and swabs from surfaces and staff members were collected. The samples were subjected to standard microbiology tests; coagulase-negative staphylococci were identified. Staphylococcus epidermidis strains were confirmed by polymerase chain reaction and subsequently tested for biofilm formation.
Coagulase-negative staphylococci were found in 81 samples, most commonly swabs from staff members. S. epidermidis accounted for 60 % of all positive results; it was most frequently isolated from surface swabs. Almost half of S. epidermidis strains were able to form biofilm. These strains were found in the environment characterized by cleanliness classes FED-STD-209E (USA) - 10 000 and FED-STD-209E (USA) - 100 000. Thus, they pose a risk for immunocompromised patients staying there. Since coagulase-negative staphylococci were also found in healthcare staff of the department, the staff members may play a key role in the transmission of these microorganisms to patients.
表皮葡萄球菌和凝固酶阴性葡萄球菌通常是医院获得性感染的重要病原体。它们形成生物膜的共同能力在这一过程中起着重要作用，生物膜是附着在惰性表面的高度有组织的微生物群落。本研究旨在确定这些细菌菌株在奥洛穆茨大学医院血液肿瘤科的流行情况及其形成生物膜的能力。
在12个月的时间里，收集空气样本、表面拭子和工作人员的拭子。对样本进行标准微生物学检测；鉴定凝固酶阴性葡萄球菌。通过聚合酶链反应确认表皮葡萄球菌菌株，随后检测其生物膜形成情况。
在81个样本中发现了凝固酶阴性葡萄球菌，最常见的是工作人员的拭子。表皮葡萄球菌占所有阳性结果的60%；最常从表面拭子中分离出来。几乎一半的表皮葡萄球菌菌株能够形成生物膜。这些菌株存在于符合美国联邦标准209E - 10000级和美国联邦标准209E - 100000级清洁度的环境中。因此，它们对住在那里的免疫功能低下患者构成风险。由于在该科室的医护人员中也发现了凝固酶阴性葡萄球菌，工作人员可能在将这些微生物传播给患者方面发挥关键作用。
