Gitay-Goren H, Kraiem Z, Lindenbaum E S
Morphological Sciences Unit, Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
Cytobios. 1988;56(225):89-99.
Morphological changes were correlated with biochemical data induced by prolactin (PRL) in cultured rat granulosa cells from large preovulatory follicles. Biochemical results indicated that PRL exerted a significant dose-dependent inhibition in gonadotrophin-induced secretion of progesterone and 17 beta-oestradiol. PRL alone failed to affect basal steroidogenic secretion. In parallel morphological experiments, using phase-contrast microscopy, untreated and 100 ng/ml PRL-treated cells appeared as a monolayer of flattened, fibroblast-like cells. Upon exposure to 0.4 IU/ml human chorionic gonadotrophin (hCG), aggregates of rounded, epithelioid-shaped cells were formed. The addition of PRL to hCG in the same doses minimized the changes induced by hCG. Similarly, electron microscopy of untreated and PRL-treated cultures revealed flat cells devoid of microvilli, with evenly dispersed microfilaments. The addition of hCG caused rounding of the cells and was accompanied by the appearance of microvilli and by pronounced steroid-producing organelles. Bundles of microfilaments were noted at the cell periphery. PRL added to hCG caused a reduction of the hCG effects, and the cell morphology was intermediate to that seen in untreated and hCG-treated cultures. The finding that PRL can prevent or minimize morphological changes caused by hCG in rat cultured granulosa cells correlates with the biochemical changes induced by PRL, and supports the concept that PRL is a modulator of gonadotrophic action in the ovary.
形态学变化与大的排卵前卵泡中培养的大鼠颗粒细胞中催乳素（PRL）诱导的生化数据相关。生化结果表明，PRL对促性腺激素诱导的孕酮和17β-雌二醇分泌具有显著的剂量依赖性抑制作用。单独的PRL未能影响基础类固醇生成分泌。在平行的形态学实验中，使用相差显微镜观察，未处理的细胞和用100 ng/ml PRL处理的细胞呈现为单层扁平的成纤维细胞样细胞。暴露于0.4 IU/ml人绒毛膜促性腺激素（hCG）后，形成了圆形的上皮样细胞聚集体。以相同剂量将PRL添加到hCG中可使hCG诱导的变化最小化。同样，未处理和PRL处理的培养物的电子显微镜检查显示，细胞扁平，无微绒毛，微丝均匀分布。添加hCG导致细胞变圆，并伴有微绒毛的出现和明显的类固醇生成细胞器。在细胞周边可见微丝束。将PRL添加到hCG中会导致hCG效应降低，细胞形态介于未处理和hCG处理的培养物之间。PRL可预防或最小化hCG在大鼠培养颗粒细胞中引起的形态学变化这一发现与PRL诱导的生化变化相关，并支持PRL是卵巢中促性腺激素作用调节剂的概念。
