膨润土衍生介孔材料固定化漆酶去除四环素。
Immobilized laccase on bentonite-derived mesoporous materials for removal of tetracycline.
机构信息
College of Environmental Science and Engineering, Hunan University and Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, PR China.
Department of Dermatology, Second Xiangya Hospital, Central South University, Changsha, 410011, PR China.
出版信息
Chemosphere. 2019 May;222:865-871. doi: 10.1016/j.chemosphere.2019.02.020. Epub 2019 Feb 6.
Bentonite is a natural and environmentally clay mineral, and bentonite-derived mesoporous materials (BDMMs) were obtained conveniently from the alkali and acid treatment of bentonite. In the present study, BDMMs were explored for immobilization of laccase obtained from Trametes versicolor. As a result, bentonite-derived mesoporous materials-Laccase (BDMMs-Lac) was developed for the removal of tetracycline (TC). The enzyme immobilization process was carried out through physical adsorption contact (ion exchange adsorption, hydrogen bond adsorption, and Van der waals adsorption) between the BDMMs and laccase. The process of immobilization remarkably increased its operating temperature. The BDMMs-Lac exhibited over 60% removal efficiency for TC within 3 h in the presence of 1-hydroxybenzotriazole (HBT). In conclusion, BDMMs-Lac showed more promising potential than free laccase for practical continuous applications.
膨润土是一种天然的环境友好型粘土矿物，通过对膨润土进行碱处理和酸处理，可以方便地得到膨润土衍生的中孔材料（BDMMs）。本研究探索了用从彩绒革盖菌中获得的漆酶对膨润土衍生的中孔材料（BDMMs）进行固定化。结果，开发了用于去除四环素（TC）的膨润土衍生的介孔材料-漆酶（BDMMs-Lac）。酶固定化过程通过 BDMMs 和漆酶之间的物理吸附接触（离子交换吸附、氢键吸附和范德华吸附）进行。固定化过程显著提高了其操作温度。在 1-羟基苯并三唑（HBT）存在的情况下，BDMMs-Lac 在 3 小时内对 TC 的去除率超过 60%。总之，BDMMs-Lac 比游离漆酶更有潜力应用于实际的连续应用。
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