Tabaeizadeh Z, Agharbaoui Z, Harrak H, Poysa V
Department of Biological Sciences, University of Quebec in Montreal, P.O. Box "8888," Station "Centre Ville," Montreal, Quebec, H3C 3P8, Canada e-mail:
Agriculture & Agri-Food Canada, Research Station, Harrow, Ontario, N0R 1G0, Canada, , , , , , CA.
Plant Cell Rep. 1999 Dec;19(2):197-202. doi: 10.1007/s002990050733.
An acidic endochitinase gene (pcht28) isolated from Lycopersicon chilense was introduced into tomato (L. esculentum) through Agrobacterium-mediated transformation, using the CAMV 35S promoter. Transgenic plants demonstrated a high level of constitutive expression of pcht28 and chitinase enzyme activity. Kanamycin-resistant R1 plants (resulting from self-pollination of transgenic plants) as well as R2 plants were evaluated for their tolerance to Verticillium dahliae (race 1 and 2 for R1 plants and race 2 for R2 plants) in the greenhouse. They demonstrated a significantly (P<0.05) higher level of tolerance to the fungi compared to the nontransgenic plants, as measured by foliar disease symptoms, vascular discoloration, and vascular discoloration index. The transgenic plants produced in this study represent a source of genetic resistance to Verticillium dahliae.
从智利番茄（Lycopersicon chilense）中分离出的酸性内切几丁质酶基因（pcht28），通过农杆菌介导的转化，利用花椰菜花叶病毒35S启动子导入番茄（L. esculentum）。转基因植物表现出pcht28的高水平组成型表达和几丁质酶活性。对卡那霉素抗性的R1植株（由转基因植物自花授粉产生）以及R2植株在温室中进行了对大丽轮枝菌的耐受性评估（R1植株针对1号和2号小种，R2植株针对2号小种）。通过叶部病害症状、维管束变色和维管束变色指数测量，与非转基因植物相比，它们对该真菌表现出显著（P<0.05）更高水平的耐受性。本研究中产生的转基因植物代表了对大丽轮枝菌的遗传抗性来源。
