Tantos Á, Mészáros A, Kissimon J, Horváth G, Farkas T
Department of Plant Physiology, University of Horticulture and Food Industry, P.O. Box 53, H-1502 Budapest, Hungary e-mail:
Institute of Biochemistry, Biological Research Center, P.O. Box 521, Szeged, Hungary, , , , , , HU.
Plant Cell Rep. 1999 Nov;19(1):88-91. doi: 10.1007/s002990050715.
Triacontanol, a long-chain primary alcohol was found to be an effective growth regulator in the micropropagation of balm, Melissa officinalis. In both the multiplication and the rooting phase, concentrations of 2, 5, 10 and 20 μg triacontanol per liter were applied. After 4 weeks of culture, the fresh weight of shoots was measured in the multiplication phase and root formation, photosynthetic activity, chlorophyll content and the fresh and dry weights of shoots were analyzed in the root induction phase. In the multiplication phase, 5 μg/l triacontanol was found to be the optimal concentration, while in the rooting phase 2 μg/l was the most effective. Triacontanol increased the number and length of roots, and it enhanced shoot growth, fresh weight, and the chlorophyll content, but it had no effect on the dry weight and the photosynthetic activity of the plants. Results of our work demonstrate that triacontanol can be applied as an effective growth regulator in the tissue culture of balm.
三十烷醇是一种长链伯醇，被发现是香蜂草（Melissa officinalis）微繁殖中一种有效的生长调节剂。在增殖和生根阶段，分别施加每升2、5、10和20微克三十烷醇的浓度。培养4周后，在增殖阶段测量芽的鲜重，在生根诱导阶段分析根的形成、光合活性、叶绿素含量以及芽的鲜重和干重。在增殖阶段，发现5微克/升的三十烷醇是最佳浓度，而在生根阶段，2微克/升最有效。三十烷醇增加了根的数量和长度，促进了芽的生长、鲜重和叶绿素含量，但对植物的干重和光合活性没有影响。我们的研究结果表明，三十烷醇可作为香蜂草组织培养中的一种有效生长调节剂。
