表达细菌非血红素氯过氧化物酶基因的转基因烟草对植物体内和体外真菌生长的抑制作用。

Inhibition of fungal growth in planta and in vitro by transgenic tobacco expressing a bacterial nonheme chloroperoxidase gene.

作者信息

Rajasekaran K, Cary J W, Jacks T J, Stromberg K D, Cleveland T E

机构信息

USDA, ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, USA Fax: +1-504-2864217 e-mail:

Department of Biology, University of Southwestern Louisiana, Lafayette, LA 70504, USA, , , , , , US.

出版信息

Plant Cell Rep. 2000 Mar;19(4):333-338. doi: 10.1007/s002990050736.

DOI:10.1007/s002990050736

PMID:30754783

Abstract

Transgenic tobacco plants producing chloroperoxidase (CPO-P), encoded by a novel gene from Pseudomonas pyrrocinia, were obtained by Agrobacterium-mediated transformation. Successful transformation was shown by PCR, Southern, northern and western blot analyses, and assays of CPO-P enzyme activity. Extracts from plants transformed with the CPO-P gene significantly reduced Aspergillus flavus colonies by up to 100% compared with extracts from control plants transformed with pBI121. Compared with controls, the transformed plants showed increased disease resistance in planta against a fungal pathogen, Colletotrichum destructivum, the causal agent of tobacco anthracnose.

摘要

通过农杆菌介导的转化获得了产生由来自吡咯假单胞菌的新基因编码的氯过氧化物酶（CPO-P）的转基因烟草植株。通过PCR、Southern、Northern和Western印迹分析以及CPO-P酶活性测定证明了成功转化。与用pBI121转化的对照植物提取物相比，用CPO-P基因转化的植物提取物可使黄曲霉菌落显著减少多达100%。与对照相比，转化植株在植株内对烟草炭疽病的病原菌——真菌病原体毁灭炭疽菌表现出增强的抗病性。