Spinelli M, Chibon P
J Biol Buccale. 1978 Mar;6(1):3-23.
Differentiation of the cells of the dental bud which produce the hard tissues of teeth was studied at the ultrastructural level; the activity of these cells (odontoblasts and ameloblasts), the laying down of organic matrices elaborated by them, the mineralization of these matrices, and the maturation of dentine and enamel are described. Injections of tritiated proline enabled us by the autoradiographic method, to confirm and to indicate more precisely the activity of these cells. Dental calcified tissues of Rana exhibit the general properties of these tissues among Vertebrates: high content of apatite, low content of organic materials (collagenous in dentine, non-collagenous in enamel), precise orientation of crystals, and true but non-prismatic enamel. The enamel of Anura as well as enamel of Urodela is a true enamel of epithelial origin.
在超微结构水平上研究了产生牙齿硬组织的牙胚细胞的分化;描述了这些细胞(成牙本质细胞和成釉细胞)的活性、它们所产生的有机基质的沉积、这些基质的矿化以及牙本质和釉质的成熟。通过放射性自显影法注射氚标记的脯氨酸,使我们能够更准确地证实并指出这些细胞的活性。蛙的牙齿钙化组织具有脊椎动物中这些组织的一般特性:磷灰石含量高,有机物质含量低(牙本质中的胶原质,釉质中的非胶原质),晶体排列精确,以及真正的但非棱柱形的釉质。无尾目动物的釉质以及有尾目动物的釉质是真正的上皮来源的釉质。