Fukuoka H, Ogawa T, Mitsuhara I, Iwai T, Isuzugawa K, Nishizawa Yoko, Gotoh Y, Nishizawa Yaeko, Tagiri A, Ugaki M, Ohshima M, Yano H, Murai N, Niwa Y, Hibi T, Ohashi Y
Chugoku National Agricultural Experiment Station, 6-12-1 Nishifukatsu, Fukuyama, Hiroshima 721-8514, Japan, , , , , , JP.
National Institute of Agrobiological Resources, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan, , , , , , JP.
Plant Cell Rep. 2000 Jul;19(8):815-820. doi: 10.1007/s002990000191.
The NCR promoter (PNCR) from soybean chlorotic mottle virus (SoyCMV) was used to express the selectable marker, neomycin phosphotransferase (nptII) gene, in Agrobacterium-mediated transformation of both monocot (rice) and dicot (tobacco) plants. A multi-cloning site for insertion of a gene of interest into the binary vector pTN is located proximal to the right border region of T-DNA. When chimeric genes under the control of other strong promoters were located in a head-to-head orientation to the PNCR-nptII gene, kanamycin-resistant tobacco shoots were generated more efficiently than when using the original pTN vectors. This suggests that the enhancer-like sequences in the promoters adjacent to PNCR may promote expression of the PNCR-nptII gene.
大豆褪绿斑驳病毒（SoyCMV）的NCR启动子（PNCR）被用于在农杆菌介导的单子叶植物（水稻）和双子叶植物（烟草）转化中表达选择标记新霉素磷酸转移酶（nptII）基因。用于将目的基因插入二元载体pTN的多克隆位点位于T-DNA右边界区域附近。当其他强启动子控制下的嵌合基因与PNCR-nptII基因呈头对头方向时，产生卡那霉素抗性烟草芽的效率比使用原始pTN载体时更高。这表明与PNCR相邻的启动子中的增强子样序列可能促进PNCR-nptII基因的表达。
