Caruso J L, Callahan J, DeChant C, Jayasimhulu K, Winget G D
Department of Biological Sciences, University of Cincinnati, P.O. Box 210006, Cincinnati, Ohio 45221-0006, USA e-mail:
Department of Environmental Health, School of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA, , , , , , US.
Plant Cell Rep. 2000 Apr;19(5):500-503. doi: 10.1007/s002990050763.
Rooted regenerated shoots obtained from leaf and nodal segments of Rosmarinus officinalis were grown on a basal nutrient medium for 9 weeks. The regenerants were shown by means of HPLC and mass spectrometry to contain carnosic acid, a diterpenoid with antioxidant and medicinal properties. Five-week-old nodular green callus also contained carnosic acid, whereas non-green, undifferentiated callus maintained in the dark did not.
从迷迭香叶段和节段获得的生根再生芽在基础营养培养基上培养9周。通过高效液相色谱法和质谱法显示，这些再生植株含有迷迭香酸，一种具有抗氧化和药用特性的二萜类化合物。五周龄的结节状绿色愈伤组织也含有迷迭香酸，而在黑暗中培养的非绿色、未分化愈伤组织则不含。
