利用绿色荧光蛋白检测转化芽和纯合后代。

Use of green fluorescent protein for detection of transformed shoots and homozygous offspring.

作者信息

Molinier J, Himber C, Hahne G

机构信息

Institut de Biologie Moléculaire des Plantes du CNRS et Université Louis Pasteur, 12, rue du Général Zimmer, F-67084 Strasbourg Cedex, France e-mail:

出版信息

Plant Cell Rep. 2000 Jan;19(3):219-223. doi: 10.1007/s002990050002.

DOI:10.1007/s002990050002

PMID:30754898

Abstract

Using tobacco as a model system, the data obtained demonstrated that the green fluorescent protein (GFP) can be used as a visual selection marker for transformed tissues. Based on differences in the intensity of GFP fluorescence, homozygous and hemizygous states could be easily visualized in seeds and seedlings of the T1 generation. These results were confirmed by genetic analysis. Optimized conditions for GFP analysis of stable transformants are presented.

摘要

以烟草作为模式系统，所获得的数据表明绿色荧光蛋白（GFP）可作为转化组织的视觉选择标记。基于GFP荧光强度的差异，在T1代种子和幼苗中可轻松观察到纯合和半合子状态。这些结果通过遗传分析得到了证实。本文还给出了稳定转化体GFP分析的优化条件。