绿色荧光蛋白作为体细胞杂交中的可视化标记
Green fluorescent protein as a visual marker in somatic hybridization.
作者信息
Olivares-Fuster O, Peña L, Duran-Vila N, Navarro L
机构信息
Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Moncada, Spain.
出版信息
Ann Bot. 2002 Apr;89(4):491-7. doi: 10.1093/aob/mcf054.
Abstract
Using a transgenic citrus plant expressing Green Fluorescent Protein (GFP) as a parent in somatic fusion experiments, we investigated the suitability of GFP as an in vivo marker to follow the processes of protoplast fusion, regeneration and selection of hybrid plants. A high level of GFP expression was detected in transgenic citrus protoplasts, hybrid callus, embryos and plants. It is demonstrated that GFP can be used for the continuous monitoring of the fusion process, localization of hybrid colonies and callus, and selection of somatic hybrid embryos and plants.
摘要
在体细胞融合实验中，我们使用表达绿色荧光蛋白（GFP）的转基因柑橘植物作为亲本，研究了GFP作为体内标记物用于追踪原生质体融合、杂种植物再生和选择过程的适用性。在转基因柑橘原生质体、杂种愈伤组织、胚和植株中检测到高水平的GFP表达。结果表明，GFP可用于连续监测融合过程、杂种菌落和愈伤组织的定位，以及体细胞杂种胚和植株的选择。
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