Schnurr J A, Guerra D J
Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA, , , , , , US.
Department of Biology, 1415 Austin Bluffs Parkway, University of Colorado, Colorado Springs, CO 80933-7150, USA e-mail:
Plant Cell Rep. 2000 Jan;19(3):279-282. doi: 10.1007/s002990050012.
The CaMV-35S promoter is one the most widely used promoters in transgenic plant research because it exhibits a high level of transcriptional activity in a variety of plant tissues. Here, the CaMV-35S promoter fused to the GUS gene was used as a model for constitutive expression in transgenic Nicotiana tabacum (cv 'Xanthi') leaves. The transgenic plants were placed under a shortened photoperiod to determine if GUS expression changed; measurements were made using fluorometry and ribonuclease protection assays. When the plants were moved from a 16 : 8-h photoperiod to a 8 : 16-h photoperiod, an increase in both specific GUS activity and gus RNA levels was observed, and these levels decreased upon returning to the 16 : 8-h photoperiod. These results indicate that photoperiod plays an important role in the regulation of the CaMV-35S promoter. Studies involving a comparison of this promoter to others should be limited to constant photoperiod conditions.
花椰菜花叶病毒35S启动子（CaMV-35S启动子）是转基因植物研究中使用最广泛的启动子之一，因为它在多种植物组织中表现出高水平的转录活性。在此，将与GUS基因融合的CaMV-35S启动子用作转基因烟草（品种‘Xanthi’）叶片组成型表达的模型。将转基因植物置于缩短的光周期下，以确定GUS表达是否发生变化；使用荧光测定法和核糖核酸酶保护测定法进行测量。当植物从16∶8小时光周期转移到8∶16小时光周期时，观察到特异性GUS活性和gus RNA水平均增加，而在恢复到16∶8小时光周期后，这些水平下降。这些结果表明光周期在CaMV-35S启动子的调控中起重要作用。涉及该启动子与其他启动子比较的研究应限于恒定光周期条件。
