a Soonchunhyang Institute of Medi-Bio Science (SIMS) , Soonchunhyang University , Cheonan-si , Chungcheongnam-do , Korea.
b Department of Embryology , Carnegie Institution for Science , Baltimore , Maryland , USA.
Nucleus. 2019 Dec;10(1):33-41. doi: 10.1080/19491034.2019.1578601.
Genome-wide mapping of lamin-B1-genome interactions has shown that gene-poor and transcriptionally inactive genomic regions are associated with the nuclear lamina. Numerous studies have suggested that lamins, the major structural components of the nuclear lamina, play a role in global chromatin organization and gene expression. How lamins could influence the 3D genome organization and transcription from the nuclear periphery has, however, remained unclear. Our recent studies showed that lamins differentially regulate distinct lamina-associated chromatin domains (LADs) at the nuclear periphery, which can in turn influence global 3D genome organization and gene expression. In this Extra View, we discuss how by using various genomics tools, it has become possible to reveal the functions of lamins in orchestrating 3D genome organization and gene expression. Abbreviations: 3D: three dimensional; LAD: lamina-associated chromatin domain; 3C: Chromosome Conformation Capture; TAD: topologically associated domain; HiLands: Histone and lamina landscape; NL: nuclear lamina; mESC: mouse embryonic stem cell; DamID: DNA adenine methyltransferase identification.
基因组范围内的 lamin-B1-基因组相互作用图谱显示，基因贫乏和转录不活跃的基因组区域与核纤层相关。许多研究表明，核纤层的主要结构成分 lamin 在全局染色质组织和基因表达中发挥作用。然而，lamin 如何影响核周的 3D 基因组组织和转录仍然不清楚。我们最近的研究表明，lamin 可差异化调节核周不同的 lamina-associated chromatin domains（LADs），这反过来又可以影响全局 3D 基因组组织和基因表达。在这个额外的视图中，我们讨论了如何使用各种基因组学工具来揭示 lamin 在协调 3D 基因组组织和基因表达中的功能。缩写：3D：三维；LAD：lamina-associated chromatin domain；3C：染色体构象捕获；TAD：拓扑相关域；HiLands：Histone 和 lamina landscape；NL：核纤层；mESC：小鼠胚胎干细胞；DamID：DNA 腺嘌呤甲基转移酶鉴定。
