Department of Botany and Microbiology, College of Sciences, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Department of Botany and Microbiology, College of Sciences, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
J Infect Public Health. 2019 Jul-Aug;12(4):549-556. doi: 10.1016/j.jiph.2019.01.065. Epub 2019 Feb 10.
Metabolites obtained from the marine microorganisms were known for their important role in microbial inhibition. Interestingly, bioprospecting of secondary metabolites from marine derived actinomycetes has huge demand especially in the treatment of multi drug resistant clinical pathogens. The present study subjected towards the identification of promising antimicrobial actinomycetes from the Arabian Gulf regions and metabolic profiling of the crude organic solvent extract by chromatographic techniques.
The strains were characterized by 16S rRNA sequencing. Extracellular metabolites were profiled by performing GC-MS analysis. MIC values of the compounds were detected using broth dilution technique.
A Gram positive, spore forming filamentous Streptomyces sp. Al-Dhabi-90 possessed good antibacterial activities against the drug resistant pathogens were confirmed by 16S rRNA gene sequencing. Further, the gas chromatography coupled with mass spectrum analysis data revealed that the organic solvent extract of the fermented Streptomyces sp. Al-Dhabi-90 contained major components such as 3-methylpyridazine, n-hexadecanoic acid, indazol-4-one, octadecanoic acid and 3a-methyl-6-((4-methylphenyl) sul respectively. The Minimum Inhibitory Concentration (MIC) of the extract against Staphylococcus aureus and Klebsiella pneumoniae were 12.5 and 50μg/ml respectively. Against drug resistant ESBL pathogens such as Escherichia coli, Pseudomonsa aeroginosa and Proteus mirabilis were 12.5, and 25μg/ml respectively. Interestingly, the extract showed promising activity against the vancomycin resistant Enterococcus faecium at 50μg/ml. The increased level of cellular constituents after the extract treatment evidenced that the metabolites altered the membrane integrity of the pathogens.
Conclusively, the marine Streptomyces sp. Al-Dhabi-90 is an ideal source for the treatment of multi drug resistant clinical pathogens.
从海洋微生物中获得的代谢产物因其在微生物抑制中的重要作用而闻名。有趣的是，特别是在治疗多药耐药临床病原体方面，从海洋衍生放线菌中生物勘探次生代谢产物的需求巨大。本研究旨在从阿拉伯湾地区鉴定有前途的抗微生物放线菌，并通过色谱技术对粗有机溶剂提取物进行代谢物分析。
通过 16S rRNA 测序对菌株进行鉴定。通过气相色谱-质谱分析（GC-MS）分析来分析细胞外代谢产物。使用肉汤稀释技术检测化合物的 MIC 值。
革兰氏阳性，孢子形成的丝状链霉菌 Al-Dhabi-90 具有良好的抗药性，对耐药病原体具有良好的抗菌活性，这是通过 16S rRNA 基因测序证实的。进一步，气相色谱-质谱联用分析数据显示，发酵的链霉菌 Al-Dhabi-90 的有机溶剂提取物含有主要成分，如 3-甲基哒嗪、正十六烷酸、吲唑-4-酮、十八烷酸和 3a-甲基-6-((4-甲基苯基)磺酰胺。提取物对金黄色葡萄球菌和肺炎克雷伯菌的最小抑菌浓度（MIC）分别为 12.5 和 50μg/ml。对多药耐药 ESBL 病原体，如大肠杆菌、铜绿假单胞菌和奇异变形杆菌，MIC 分别为 12.5 和 25μg/ml。有趣的是，该提取物对万古霉素耐药粪肠球菌（Enterococcus faecium）表现出有希望的活性，MIC 为 50μg/ml。提取物处理后细胞成分水平升高，证明代谢物改变了病原体的膜完整性。
综上所述，海洋链霉菌 Al-Dhabi-90 是治疗多药耐药临床病原体的理想来源。
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