Izhar Syed Khalida, Bano Naushin, Rizvi Shareen Fatima, Gupta Anmol, Pandey Pratibha, Lakhanpal Sorabh, Singh Ajay, Khan Fahad, Upadhye Vijay Jagdish, Afaq Uzma
Department of Biosciences, Faculty of Science, Integral University, Kursi Road, Lucknow, India.
University Centre for Research and Development,, Chandigarh University, Gharuan, Mohali, 140413, Punjab, India.
Sci Rep. 2025 Aug 27;15(1):31540. doi: 10.1038/s41598-025-15657-7.
Parthenium hysterophorus, identified as Congress weed, presents a considerable risk to agricultural ecosystems and human health owing to its invasive characteristics and allelopathic properties. In this study, we explore the potential of biosynthesized silver nanoparticles (AgNPs) using actinobacteria as a sustainable and effective product for inhibiting the growth of P. hysterophorus. Actinobacteria, known for their diverse metabolic capabilities, were isolated and utilized for the eco-friendly synthesis of AgNPs. The reaction mixture consisted of an aqueous silver nitrate solution (1 mM) and the cell-free culture supernatant of Actinobacteria, which served as a source of reducing and stabilizing metabolites for the biosynthesis of silver nanoparticles. The resultant AgNPs were characterised employing several analytical techniques, such as UV-Vis spectroscopy and transmission electron microscopy. The synthesized AgNPs exhibited distinct physicochemical properties such as a surface plasmon resonance peak at ~ 420 nm, spherical shape, nanoscale size of 20.2 nm, confirming their successful formation. Subsequently, the inhibitory activity of actinobacteria-synthesized AgNPs against P. hysterophorus was evaluated through comprehensive bioassays. The nanoparticles demonstrated remarkable inhibition of seed germination, seedling growth, and overall plant development. Moreover, the AgNPs displayed selective toxicity towards Parthenium weed while maintaining minimal impact on non-target plants, emphasizing their potential as a targeted and environmentally friendly solution. The study highlights the promising role of actinobacteria-synthesized AgNPs in managing P. hysterophorus, providing valuable insights into the development of sustainable strategies for weed control. This research contributes to the growing field of nanobiotechnology, showcasing the potential of bioengineered nanoparticles as an effective means to address invasive plant species and promote sustainable agricultural practices.
银胶菊，被认定为大黍，因其入侵特性和化感作用，对农业生态系统和人类健康构成了相当大的风险。在本研究中，我们探索了利用放线菌生物合成银纳米颗粒（AgNPs）作为一种可持续且有效的产品来抑制银胶菊生长的潜力。放线菌以其多样的代谢能力而闻名，被分离出来用于银纳米颗粒的生态友好型合成。反应混合物由硝酸银水溶液（1 mM）和放线菌的无细胞培养上清液组成，后者作为银纳米颗粒生物合成的还原和稳定代谢物来源。所得的银纳米颗粒采用多种分析技术进行表征，如紫外可见光谱和透射电子显微镜。合成的银纳米颗粒表现出独特的物理化学性质，如在约420 nm处有表面等离子体共振峰、球形形状、20.2 nm的纳米级尺寸，证实了它们的成功形成。随后，通过全面的生物测定评估了放线菌合成的银纳米颗粒对银胶菊的抑制活性。这些纳米颗粒对种子萌发、幼苗生长和整体植物发育表现出显著的抑制作用。此外，银纳米颗粒对银胶菊显示出选择性毒性，同时对非目标植物的影响最小，强调了它们作为一种有针对性且环境友好的解决方案的潜力。该研究突出了放线菌合成的银纳米颗粒在管理银胶菊方面的潜在作用，为杂草控制可持续策略的发展提供了有价值的见解。这项研究为纳米生物技术这一不断发展的领域做出了贡献，展示了生物工程纳米颗粒作为解决入侵植物物种和促进可持续农业实践的有效手段的潜力。
