Jiang Wenqian, Tian Yarong, Zuo Rui, Lin Jun
Institute of Applied Genomics, Fuzhou University, Fuzhou 350108, Fujian, China.
College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, Fujian, China.
Sheng Wu Gong Cheng Xue Bao. 2019 Jan 25;35(1):27-39. doi: 10.13345/j.cjb.180102.
Basic research in life science and medicine has dug into single cell level in recent years. Single-cell analysis offers to understand life from diverse perspectives and is used to profile cell heterogeneity to investigate mechanism of diseases. Single cell technologies have also found applications in forensic medicine and clinical reproductive medicine, while the techniques are rapidly evolving and have become more and more sophisticated. In this article, we reviewed various single cell isolation techniques and their pros and cons, including manual cell picking, laser capture microdissection and microfluidics, as well as analysis methods for DNA, RNA and protein in single cell. In addition, we summarized major up-to-date single cell research achievements and their potential applications.
近年来，生命科学和医学的基础研究已深入到单细胞水平。单细胞分析有助于从不同角度理解生命，并用于描绘细胞异质性以研究疾病机制。单细胞技术也已在法医学和临床生殖医学中得到应用，同时这些技术正在迅速发展且变得越来越复杂。在本文中，我们综述了各种单细胞分离技术及其优缺点，包括手动细胞挑选、激光捕获显微切割和微流控技术，以及单细胞中DNA、RNA和蛋白质的分析方法。此外，我们总结了最新的主要单细胞研究成果及其潜在应用。
