Osband M, Parkman R
J Immunol. 1978 Jul;121(1):179-85.
To determine if autocytotoxic lymphocytes were naturally occurring in murine spleens, C57BL/6 spleen cells were fractionated on discontinuous BSA gradients. Autocytotoxicity was assessed in vitro (cytotoxicity of C57BL/6 fibroblasts) and in vivo (splenomegaly and popliteal node enlargement in C57BL/6 mice). A medium density subpopulation of lymphocytes was shown to be autocytotoxic and to be similar to autoreactive lymphocytes produced by the in vitro "sensitization" of splenic lymphocytes on syngeneic fibroblasts monolayers. The naturally occurring autocytotoxic lymphocytes express no detectable theta antigen, did not adhere to nylon, but did have an Fc receptor. In recombination experiments, BSA-fractionated lymphocytes were incubated with autocytotoxic and "sensitized" lymphocytes. A light density subpopulation was shown to suppress both autoreactive lymphocyte subpopulations at a 1:50 ratio. The suppressor cells were nylon nonadherent T lymphocytes. The lack of autoreactivity of unfractionated murine spleen cells is due to the concomitant presence of autocytotoxic and suppressor lymphocytes. If suppressor lymphocytes are selectively removed in vitro, the reactivity of autocytotoxic lymphocytes can be detected.
为了确定自细胞毒性淋巴细胞是否天然存在于小鼠脾脏中，将C57BL/6脾细胞在不连续的牛血清白蛋白梯度上进行分级分离。在体外（C57BL/6成纤维细胞的细胞毒性）和体内（C57BL/6小鼠的脾肿大和腘淋巴结肿大）评估自细胞毒性。结果显示，中等密度的淋巴细胞亚群具有自细胞毒性，并且类似于通过脾淋巴细胞在同基因成纤维细胞单层上进行体外“致敏”产生的自身反应性淋巴细胞。天然存在的自细胞毒性淋巴细胞不表达可检测到的θ抗原，不粘附于尼龙，但具有Fc受体。在重组实验中，将经牛血清白蛋白分级分离的淋巴细胞与自细胞毒性淋巴细胞和“致敏”淋巴细胞一起孵育。结果显示，低密度亚群以1:50的比例抑制两种自身反应性淋巴细胞亚群。抑制细胞是不粘附尼龙的T淋巴细胞。未分级的小鼠脾细胞缺乏自身反应性是由于自细胞毒性淋巴细胞和抑制性淋巴细胞同时存在。如果在体外选择性去除抑制性淋巴细胞，则可以检测到自细胞毒性淋巴细胞的反应性。
