分枝杆菌在模拟巨噬细胞吞噬体的环境中释放的膜囊泡的特征。
Characterization of membrane vesicles released by Mycobacterium avium in response to environment mimicking the macrophage phagosome.
机构信息
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA.
Department of Microbiology, College of Science, Oregon State University, Corvallis, OR 97331, USA.
出版信息
Future Microbiol. 2019 Mar;14(4):293-313. doi: 10.2217/fmb-2018-0249. Epub 2019 Feb 13.
AIM
To investigate the formation of Mycobacterium avium membrane vesicles (MVs) within macrophage phagosomes.
MATERIALS & METHODS: A phagosome model was utilized to characterize proteomics and lipidomics of MVs. A click chemistry-based enrichment assay was employed to examine the presence of MV proteins in the cytosol of host cells.
RESULTS
Exposure to metals at concentrations present in phagosomes triggers formation of bacterial MVs. Proteomics identified several virulence factors, including enzymes involved in the cell wall synthesis, lipid and fatty acid metabolism. Some of MV proteins were also identified in the cytosol of infected macrophages. MVs harbor dsDNA.
CONCLUSION
M. avium produces MVs within phagosomes. MVs carry products with potential roles in modulation of host immune defenses and intracellular survival.
目的
研究分枝杆菌属囊泡（MVs）在巨噬细胞吞噬体中的形成。
材料与方法
利用吞噬体模型来描述分枝杆菌属 MVs 的蛋白质组学和脂质组学。采用基于点击化学的富集分析方法来检测宿主细胞细胞质中 MV 蛋白的存在。
结果
在吞噬体中存在的浓度的金属暴露会触发细菌 MVs 的形成。蛋白质组学鉴定出了几种毒力因子，包括参与细胞壁合成、脂质和脂肪酸代谢的酶。一些 MV 蛋白也在感染的巨噬细胞的细胞质中被鉴定出来。MVs 携带双链 DNA。
结论
分枝杆菌属在吞噬体中产生 MVs。MVs 携带具有调节宿主免疫防御和细胞内生存能力的潜在作用的产物。
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