Devey M E, Lee S R, Le Page S, Feldman R, Isenberg D A
Department of Medical Microbiology, London School of Hygiene and Tropical Medicine, UK.
J Autoimmun. 1988 Oct;1(5):483-94. doi: 10.1016/0896-8411(88)90069-8.
The functional affinity and IgG subclass of antibodies to ss and dsDNA were measured by ELISA in five serial samples from 41 patients with systemic lupus erythematosus (SLE) who were divided into relatively homogeneous disease subgroups. Anti-dsDNA antibodies were restricted to IgG1 and IgG3 in renal disease and levels increased with disease severity. Functional affinity of IgG1 and IgG3 anti-dsDNA fell in patients with severe renal disease, suggesting that the high affinity antibody population lost from the serum was localizing in the kidneys. IgG2 anti-dsDNA were found in patients with joint and skin disease alone and in the thrombotic/spontaneous abortion subgroup. IgG2 antibody levels did not correlate with disease severity but did correlate with the presence of antibodies to Klebsiella K30 and may have represented a cross-reactive antibody population.
通过酶联免疫吸附测定法（ELISA）测量了41例系统性红斑狼疮（SLE）患者五个连续样本中抗单链和双链DNA抗体的功能亲和力及IgG亚类，这些患者被分为相对同质的疾病亚组。在肾脏疾病中，抗双链DNA抗体局限于IgG1和IgG3，且水平随疾病严重程度增加。重症肾脏疾病患者中IgG1和IgG3抗双链DNA的功能亲和力下降，这表明从血清中丢失的高亲和力抗体群体定位于肾脏。仅在关节和皮肤疾病患者以及血栓形成/自然流产亚组中发现了IgG2抗双链DNA。IgG2抗体水平与疾病严重程度无关，但与抗肺炎克雷伯菌K30抗体的存在相关，可能代表了一种交叉反应性抗体群体。
