Department of Biology, Johns Hopkins University, Baltimore, Maryland.
Endocrinology. 2019 Apr 1;160(4):782-790. doi: 10.1210/en.2018-00985.
Tamoxifen, a selective estrogen-receptor modulator, is widely used in mouse models to temporally control gene expression but is also known to affect body composition. We report that tamoxifen has significant and sustained effects on glucose tolerance, independent of effects on insulin sensitivity, in mice. IP, but not oral, tamoxifen delivery improved glucose tolerance in three inbred mouse strains. The extent and persistence of tamoxifen-induced effects were sex and strain dependent. These findings highlight the need to revise commonly used tamoxifen-based protocols for gene manipulation in mice by including longer chase periods after injection, oral delivery, and the use of tamoxifen-treated littermate controls.
他莫昔芬是一种选择性雌激素受体调节剂，广泛用于小鼠模型中以暂时控制基因表达，但也已知会影响身体成分。我们报告称，他莫昔芬对葡萄糖耐量有显著且持续的影响，而与胰岛素敏感性无关，在小鼠中。IP，但不是口服，他莫昔芬给药改善了三种近交系小鼠的葡萄糖耐量。他莫昔芬诱导作用的程度和持久性与性别和品系有关。这些发现强调需要修改常用的基于他莫昔芬的基因操作方案，包括在注射后更长的追踪期、口服给药以及使用他莫昔芬处理的同窝对照。
