Cho H S, Cao J, Ren J P, Earle E D
Department of Plant Breeding, Cornell University, Ithaca, NY 14853-1901, USA e-mail:
Plant Cell Rep. 2001 Jan;20(1):1-7. doi: 10.1007/s002990000278.
A synthetic Bacillus thuringiensis cry1C gene was transferred to three Korean cultivars of Chinese cabbage via Agrobacterium tumefaciens-mediated transformation of hypocotyl explants. Hygromycin resistance served as an efficient selective marker. The transformation efficiency ranged from 5% to 9%. Transformation was confirmed by Southern blot analysis, PCR, Northern analysis, and progeny tests. Many transgenic plants of the closed-head types (lines Olympic and Samjin) flowered in vitro. Over 50 hygromycin-resistant plants were successfully transferred to soil. The transgenic plants and their progeny were resistant to diamondback moths (DBM, Plutella xylostella), the major insect pest of crucifers world-wide, as well as to cabbage loopers (Trichoplusia ni) and imported cabbage worms (Pieris rapae). Both susceptible Geneva DBM and a DBM population resistant to Cry1A protein were controlled by the Cry1C-transgenic plants. The efficient and reproducible transformation system described may be useful for the transfer of other agriculturally important genes into Chinese cabbage.
通过根癌农杆菌介导的下胚轴外植体转化，将一个人工合成的苏云金芽孢杆菌cry1C基因导入了三个韩国大白菜品种。潮霉素抗性作为一种有效的选择标记。转化效率在5%至9%之间。通过Southern杂交分析、PCR、Northern分析和后代测试对转化进行了确认。许多闭头型转基因植株（Olympic和Samjin品系）在离体条件下开花。50多株潮霉素抗性植株成功移栽到土壤中。转基因植株及其后代对小菜蛾（十字花科植物在全球范围内的主要害虫）、甘蓝夜蛾和菜青虫具有抗性。Cry1C转基因植株既能控制敏感的日内瓦小菜蛾种群，也能控制对Cry1A蛋白具有抗性的小菜蛾种群。所描述的高效且可重复的转化系统可能有助于将其他农业上重要的基因导入大白菜。
