携带来自苏云金芽孢杆菌的合成cry1Ia8基因的转基因甘蓝植株在田间条件下对两种鳞翅目昆虫具有高抗性。

High resistance of transgenic cabbage plants with a synthetic cry1Ia8 gene from Bacillus thuringiensis against two lepidopteran species under field conditions.

作者信息

Yi Dengxia, Yang Weijie, Tang Jun, Wang Li, Fang Zhiyuan, Liu Yumei, Zhuang Mu, Zhang Yangyong, Yang Limei

机构信息

Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.

Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

出版信息

Pest Manag Sci. 2016 Feb;72(2):315-21. doi: 10.1002/ps.3994. Epub 2015 Apr 8.

DOI:10.1002/ps.3994

PMID:25721816

Abstract

BACKGROUND

Plutella xylostella (Linnaeus) has become the most destructive pest in cabbage throughout the world. Cry1Ia8 cabbage has been developed to reduce pest attacks. To gain a better understanding of the efficacy of Cry1Ia8 cabbage, a homozygous Cry1Ia8 cabbage line A14-5 was produced, and its resistance to P. xylostella, Pieris rapae (Linnaeus) and other lepidopteran pests was evaluated in the field in 2011, 2012 and 2013.

RESULTS

Under natural infestation conditions, the homozygous transgenic line was highly resistant against P. xylostella and P. rapae as compared with the untransformed control and susceptible to Mamestra brassicae (Linnaeus) and Spodoptera exigua (Hübner). The homozygous transgenic plants showed slight symptoms of damaged leaves by lepidopteran species, while the untransformed plants exhibited serious damage symptoms throughout the cabbage growing season.

CONCLUSION

Compared with the control, the homozygous transgenic cabbage line showed great potential for protecting cabbage from attack by P. xylostella and P. rapae in the field.

摘要