Prasad P, Savyasachi S, Reddy L Prasanna Anjaneya, Sreedhar R V
Department of Lipid Science, CSIR-Central Food Technological Research Institute (CSIR-CFTRI).
Academy of Scientific and Innovative Research (AcSIR), CSIR-Central Food Technological Research Institute campus.
J Oleo Sci. 2019 Mar 1;68(3):209-223. doi: 10.5650/jos.ess18219. Epub 2019 Feb 13.
Buglossoides arvensis is indigenous to India and its seed oil is rich in unique and nutritionally important omega-3 fatty acid namely, stearidonic acid (SDA). It is a non-conventional oil seed plant and needs to be agronomically adapt for commercial utilization. In the present study, oil extracted from the agronomically adapted high yielding cultivar of B. arvensis seeds was analyzed for its oil content, fatty acid (FA) composition, physico-chemical characteristics, total lipids and triacylglycerol molecular species. The oil content, peroxide, acid, iodine, p-anisidine values and tocopherol content of the oil were 18.53% (w/w), 2.06 meq of active oxygen / kg of oil, 2.55 mg KOH/g oil, 217.2 g I/100g oil, 10.7 and 774.8 mg/kg oil respectively. Oxidative stability as determined by the induction period was found to be 3.1 h. Polyunsaturated fatty acid (PUFA) content of the oil was 81.3% (of total FA), comprising of α-linolenic acid (ALA; 48.5%), SDA (18.6%), linoleic acid (LA; 10.3%) and γ-linolenic acid (GLA; 3.9%). Profiling of lipid classes showed neutral lipids (89.3%, w/w) as most abundant lipid class followed by glycolipids (7.4%, w/w) and phospholipids (3.3%, w/w). High resolution mass spectrometric analysis of triacylglycerol (TAG) molecular species showed TAGs with C54 carbons in the acyl chain as most abundant. Positional distribution analysis showed GLA and SDA predominantly at the sn-2 position of triacylglycerol. FTIR analysis revealed common characteristics molecular features similar to PUFA rich oils. Overall, the results suggest that B. arvensis seed oil is an excellent ω3-ω6-ω3 or ALA-SDA-GLA source for food and nutraceutical industries.
原野勿忘草原产于印度，其种子油富含独特且具有重要营养意义的ω-3脂肪酸，即硬脂酸（SDA）。它是一种非常规油料作物，需要在农艺学上进行适应性改良以实现商业利用。在本研究中，对从农艺学适应性良好的高产原野勿忘草品种种子中提取的油进行了油含量、脂肪酸（FA）组成、物理化学特性、总脂质和三酰甘油分子种类分析。该油的油含量、过氧化物、酸值、碘值、对茴香胺值和生育酚含量分别为18.53%（w/w）、2.06 meq活性氧/千克油、2.55 mg KOH/克油、217.2 g I/100克油、10.7和774.8 mg/千克油。通过诱导期测定的氧化稳定性为3.1小时。该油的多不饱和脂肪酸（PUFA）含量为81.3%（占总FA），由α-亚麻酸（ALA；48.5%）、SDA（18.6%）、亚油酸（LA；10.3%）和γ-亚麻酸（GLA；3.9%）组成。脂质类别的分析表明，中性脂质（89.3%，w/w）是最丰富的脂质类别，其次是糖脂（7.4%，w/w）和磷脂（3.3%，w/w）。三酰甘油（TAG）分子种类的高分辨率质谱分析表明，酰基链中含54个碳的TAG最为丰富。位置分布分析表明，GLA和SDA主要位于三酰甘油的sn-2位置。傅里叶变换红外光谱（FTIR）分析揭示了与富含PUFA的油相似的共同特征分子特征。总体而言，结果表明原野勿忘草种子油是食品和营养保健品行业优质的ω3-ω6-ω3或ALA-SDA-GLA来源。
