Kurvits Lille, Reimann Ene, Kadastik-Eerme Liis, Truu Laura, Kingo Külli, Erm Triin, Kõks Sulev, Taba Pille, Planken Anu
Department of Neurology and Neurosurgery, University of Tartu, Tartu, Estonia.
Department of Neurology, Charité - Universitätsmedizin Berlin, Berlin, Germany.
Front Neurosci. 2019 Jan 29;13:13. doi: 10.3389/fnins.2019.00013. eCollection 2019.
We report the changed levels of serum amyloid alpha, an immunologically active protein, in Parkinson's disease (PD) patients' peripheral tissues. We have previously shown that and (serum amyloid alpha-1,-2, genes) were among the top downregulated genes in PD patients' skin, using whole-genome RNA sequencing. In the current study, we characterized the gene and protein expression profiles of skin and blood samples from patients with confirmed PD diagnosis and age/sex matched controls. qRT-PCR analysis of PD skin demonstrated downregulation of and genes in PD patients. However, the lowered amount of protein could not be visualized using immunohistochemistry, due to low quantity of SAA (Serum Amyloid Alpha, protein) in skin. and expression levels in whole blood were below detection threshold based on RNA sequencing, however significantly lowered protein levels of SAA1/2 in PD patients' serum were shown with ELISA, implying that SAA is secreted into the blood. These results show that SAA is differentially expressed in the peripheral tissues of PD patients.
我们报告了帕金森病（PD）患者外周组织中具有免疫活性的蛋白质血清淀粉样蛋白α水平的变化。我们之前通过全基因组RNA测序表明，SAA1和SAA2（血清淀粉样蛋白α-1、-2基因）是PD患者皮肤中下调最明显的基因之一。在本研究中，我们对确诊为PD的患者以及年龄/性别匹配的对照者的皮肤和血液样本的基因和蛋白质表达谱进行了表征。对PD患者皮肤进行qRT-PCR分析显示，PD患者中SAA1和SAA2基因表达下调。然而，由于皮肤中血清淀粉样蛋白α（SAA，蛋白质）含量较低，使用免疫组织化学无法观察到蛋白质含量的降低。基于RNA测序，全血中的SAA1和SAA2表达水平低于检测阈值，然而，ELISA显示PD患者血清中SAA1/2的蛋白质水平显著降低，这意味着SAA分泌到了血液中。这些结果表明，SAA在PD患者的外周组织中存在差异表达。
