乙二胺四乙酸和生物炭对反枝苋体内铜积累和亚细胞分布的影响
Effect of ethylenediaminetetraacetic acid and biochar on Cu accumulation and subcellular partitioning in Amaranthus retroflexus L.
机构信息
College of Environmental and Resource Sciences, Shanxi University, Taiyuan, 030000, China.
Environment Research Institute, Shandong University, Qingdao, 266237, China.
出版信息
Environ Sci Pollut Res Int. 2019 Apr;26(10):10343-10353. doi: 10.1007/s11356-019-04448-z. Epub 2019 Feb 13.
Phytoremediation combined with amendments and stabilization technologies are two crucial methods to deal with soil contaminated with heavy metals. Copper (Cu) contamination in soil near Cu mines poses a serious threat to ecosystems and human health. This study investigated the effect of ethylenediaminetetraacetic acid (EDTA) and biochar (BC) on the accumulation and subcellular distribution of Cu in Amaranthus retroflexus L. to demonstrate the remediation mechanism of EDTA and BC at the cellular level. The role of calcium (Ca) in response to Cu stress in A. retroflexus was also elucidated. We designed a pot experiment with a randomized block of four Cu levels (0, 100, 200, 400 mg kg) and three treatments (control, amendment with EDTA, and amendment with BC). The subcellular components were divided into three parts (cell walls, organelles, and soluble fraction) by differential centrifugation. The results showed that EDTA amendment significantly increased (p < 0.05) the concentrations of Cu in root cell walls and all subcellular components of stems and leaves (cell walls, organelles, and the soluble fraction). EDTA amendment significantly increased (p < 0.05) the proportion of exchangeable fraction and carbonate fraction in the soil. While BC amendment significantly decreased (p < 0.05) the concentrations of Cu in root cell walls and the root soluble fraction, it had no significant effects on Cu concentrations in the subcellular components of stems and leaves. The results revealed that EDTA mainly promoted the transfer of Cu to aboveground parts and accumulation in subcellular components of stems and leaves, while BC mainly limited Cu accumulation in root cell walls and the root soluble fraction. Ca concentrations in cell walls of roots, stems, and leaves increased as the Cu stress increased in all treatment groups, indicating that Ca plays an important role in relieving Cu toxicity in Amaranthus retroflexus L.
植物修复与添加改良剂和稳定化技术相结合是处理重金属污染土壤的两种重要方法。铜矿附近土壤中的铜污染对生态系统和人类健康构成了严重威胁。本研究探讨了乙二胺四乙酸（EDTA）和生物炭（BC）对苋菜（Amaranthus retroflexus L.）中铜积累和亚细胞分布的影响，以证明 EDTA 和 BC 在细胞水平上的修复机制。还阐明了钙（Ca）在苋菜应对铜胁迫中的作用。我们设计了一个随机区组的盆栽实验，有四个铜水平（0、100、200、400mgkg）和三个处理（对照、EDTA 改良剂和 BC 改良剂）。通过差速离心将亚细胞成分分为细胞壁、细胞器和可溶部分三个部分。结果表明，EDTA 改良剂显著增加（p<0.05）了根细胞壁和茎叶所有亚细胞成分（细胞壁、细胞器和可溶部分）中铜的浓度。EDTA 改良剂显著增加（p<0.05）了土壤中可交换态和碳酸盐态铜的比例。而 BC 改良剂显著降低（p<0.05）了根细胞壁和根可溶部分中铜的浓度，但对茎叶亚细胞成分中铜的浓度没有显著影响。结果表明，EDTA 主要促进了 Cu 向地上部分的转移和积累在茎叶的亚细胞成分中，而 BC 主要限制了 Cu 在根细胞壁和根可溶部分的积累。所有处理组中，Cu 胁迫下根、茎和叶细胞壁中的 Ca 浓度增加，表明 Ca 在缓解苋菜 Cu 毒性中起着重要作用。
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