Subcellular Compartmentalization and Chemical Forms of Lead Participate in Lead Tolerance of L. with .

The effect of arbuscular mycorrhizal fungus on the subcellular compartmentalization and chemical forms of lead (Pb) in Pb tolerance plants was assessed in a pot experiment in greenhouse conditions. We measured root colonization, plant growth, photosynthesis, subcellular compartmentalization and chemical forms of Pb in black locust ( L.) seedlings inoculated with isolate (BGC XJ01A) under a range of Pb treatments (0, 90, 900, and 3000 mg Pb kg soil). The majority of Pb was retained in the roots of under Pb stress, with a significantly higher retention in the inoculated seedlings. inoculation significantly increased the proportion of Pb in the cell wall and soluble fractions and decreased the proportion of Pb in the organelle fraction of roots, stems, and leaves, with the largest proportion of Pb segregated in the cell wall fraction. inoculation increased the proportion of inactive Pb (especially pectate- and protein-integrated Pb and Pb phosphate) and reduced the proportion of water-soluble Pb in the roots, stems, and leaves. The subcellular compartmentalization of Pb in different chemical forms was highly correlated with improved plant biomass, height, and photosynthesis in the inoculated seedlings. This study indicates that could improve Pb tolerance in seedlings growing in Pb polluted soils.