Huang Li, Zhang Haoqiang, Song Yingying, Yang Yurong, Chen Hui, Tang Ming
College of Life Sciences, Northwest A&F UniversityYangling, China.
College of Forestry, Northwest A&F UniversityYangling, China.
Front Plant Sci. 2017 Apr 10;8:517. doi: 10.3389/fpls.2017.00517. eCollection 2017.
The effect of arbuscular mycorrhizal fungus on the subcellular compartmentalization and chemical forms of lead (Pb) in Pb tolerance plants was assessed in a pot experiment in greenhouse conditions. We measured root colonization, plant growth, photosynthesis, subcellular compartmentalization and chemical forms of Pb in black locust ( L.) seedlings inoculated with isolate (BGC XJ01A) under a range of Pb treatments (0, 90, 900, and 3000 mg Pb kg soil). The majority of Pb was retained in the roots of under Pb stress, with a significantly higher retention in the inoculated seedlings. inoculation significantly increased the proportion of Pb in the cell wall and soluble fractions and decreased the proportion of Pb in the organelle fraction of roots, stems, and leaves, with the largest proportion of Pb segregated in the cell wall fraction. inoculation increased the proportion of inactive Pb (especially pectate- and protein-integrated Pb and Pb phosphate) and reduced the proportion of water-soluble Pb in the roots, stems, and leaves. The subcellular compartmentalization of Pb in different chemical forms was highly correlated with improved plant biomass, height, and photosynthesis in the inoculated seedlings. This study indicates that could improve Pb tolerance in seedlings growing in Pb polluted soils.
在温室条件下进行的盆栽试验中，评估了丛枝菌根真菌对耐铅植物中铅（Pb）的亚细胞区室化和化学形态的影响。我们测定了在一系列铅处理（0、90、900和3000 mg Pb kg土壤）下，接种分离株（BGC XJ01A）的刺槐（Robinia pseudoacacia L.）幼苗的根系定殖、植物生长、光合作用、铅的亚细胞区室化和化学形态。在铅胁迫下，大部分铅保留在刺槐的根系中，接种幼苗中的保留量显著更高。接种显著增加了根、茎和叶中细胞壁和可溶性部分中铅的比例，降低了细胞器部分中铅的比例，其中细胞壁部分中铅的比例最大。接种增加了根、茎和叶中无活性铅（特别是果胶和蛋白质结合的铅以及磷酸铅）的比例，降低了水溶性铅的比例。接种幼苗中不同化学形态铅的亚细胞区室化与植物生物量、株高和光合作用的改善高度相关。本研究表明，接种丛枝菌根真菌可以提高在铅污染土壤中生长的刺槐幼苗的耐铅性。
